摘要
目的探讨寻常性银屑病患者皮损中白介素17(IL-17)、干扰素γ(IFN-γ)以及巨噬细胞炎性蛋白3α(MIP-3α)mRNA的表达与发病的关系。方法用逆转录-聚合酶链反应(RT-PCR)方法检测了31例寻常性银屑病患者皮损和16例正常人皮肤中IL-17、IFN-γ和MIP-3αmRNA的表达。结果寻常性银屑病患者皮损及正常人皮肤组织中均有IL-17、IFN-γ和MIP-3αmRNA的表达。在寻常性银屑病患者皮损中IL-17mRNA的表达水平为1.142±0.059(A值,下同),较正常人对照组(0.879±0.034)明显增高(P<0.001);IFN-γmRNA的表达水平在皮损和正常人对照分别为1.114±0.056和0.905±0.026,差异有显著性(P<0.001);MIP-3αmRNA的表达水平为1.140±0.052,亦明显高于正常人对照组(0.868±0.031)(P<0.001)。结论寻常性银屑病患者皮损中IL-17、IFN-γ及MIP-3αmRNA表达水平的上调可能与银屑病的发病相关。
Objective To investigate the expression levels of of interleukin-17(IL-17),interferon-gamma(IFN-γ),and macrophage inflammatory protein-3alpha(MIP-3α)mRNA in skin lesions of pa-tients with psoriasis vulgaris.Methods The skin biopsies were collected from skin lesions in31cases of psoriasis vulgaris and16normal controls.The expressions of IL-17,IFN-γ,and MIP-3αmRNA were semi-quantitatively analyzed with reverse transcriptase-polymerase chain reaction(RT-PCR)in psoriatic lesions and normal skin tissues.Results Expressions of IL-17,IFN-γ,and MIP-3αwere presented in all speci-mens of both psoriatic lesions and normal controls.The expression levels were1.142±0.059for IL-17mRNA,1.114±0.056for IFN-γmRNA,1.140±0.052for MIP-3αmRNA,in skin lesions,respectively,which were greatly higher than those in normal controls(0.879±0.034,0.905±0.026and0.868±0.031,respectively)(all P <0.001).Conclusion The results indicate that up-regulated expression of IL-17,IFN-γand MIP-3αmRNA might be related to the pathogenesis of psoriasis.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2004年第3期150-152,共3页
Chinese Journal of Dermatology
