摘要
目的 探讨斑块状银屑病患者表皮干扰素-γ受体mRNA的表达及其在发病机制中的作用。方法 采集28例斑块状银屑病患者皮损及周围外观正常皮肤。28例正常人皮肤作为对照，分离表皮，逆转录聚合酶链反应（RT-PCR）法检测干扰素-γ受体mRNA表达水平。PASI评分法评估银屑病的严重程度。结果 斑块状银屑病患者皮损和非皮损表皮干扰素-γ受体mRNA的表达率为100％，对照样本的表达率为17．86％（5／28）；患者皮损、非皮损和对照表皮干扰素-γ受体mRNA的表达水平均值分别为1．002±0．563、0．188±0、095、0．005±0．012，皮损处的表达水平明显高于非皮损处（t=7．54，P〈0、01）。非皮损处明显高于正常人对照；进行期与稳定期皮损的表达水平分别为1、210±0．489和0．379±0．163，进行期显著高于稳定期（t=4．37，P〈0．01）；而皮损处的表达水平与PASI评分值无相关性。结论 表皮干扰素-γ受体mRNA的表达可能与银屑病皮损的形成和病情的活动性有关。
Objective To explore the-mRNA expression of IFN-gamma receptor in epidermis of patients with plaque psoriasis and its role in the pathogenesis of psoriasis. Methods Lesional and non-lesional specimens were obtained from psoriatic patients （ n = 28 ） and normal controls （ n = 28 ）. Epidermis was detached, and subjected to reverse transcriptase polymerase chain reaction （RT-PCR） for the measurement of IFN-gamma receptor mRNA. The disease severity was evaluated by psoriasis area and severity index （PASI）. Results The IFN-gamma receptor mRNA was expressed in all lesional and non-lesional epidermis from the patients, while in only 17.86% （ 5/28 ） from controls. The mean value of IFN-gamma receptor mRNA in the lesional epidermis was significantly higher than that in the non-lesional epidermis （ 1.002±0.563 vs 0.188 ± 0.095, t = 7.54, P 〈 0.01 ）, and the latter was significantly elevated compared with the controls （ 0.188 ± 0.095 vs 0.005 ± 0.012, t= 10.17, P〈 0.01 ）. The expression level of IFN-gamma receptor mRNA was significantly higher in active lesions than in stable lesions （ 1.210 ± 0.489 vs 0.379 ± 0.163, t= 4.37, P〈 0.01 ）. However, no correlation was found between the expression levels of IFN-gamma receptor mRNA and disease severity. Conclusions The mRNA expression of IFN-gamma receptor may be related to the lesion formation and disease severity in psoriatic patients.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2007年第3期146-148,共3页
Chinese Journal of Dermatology
基金
江苏省人事厅“六大人才高峰”项目资助,江苏省自然科学基金部分资助（BK2001195）,国家自然科学基金部分资助（30170863）
关键词
银屑病
受体
干扰素
Psoriasis
Receptor, interferon
