摘要
本实验研究表明,Iq7613在体外条件下,可直接抑制人宫颈癌Hela细胞的DNA合成,并且随着药物浓度的增加及作用时间的延长,抑制作用更为明显。采用显微分光光度术检测Iq7613对小鼠S_(180)腹水瘤细胞核DNA的影响,结果显示:用药后4小时即出现G_1期细胞大量堆积,进入S期完全受阻。用药后24小时仍有较多细胞堆积于G_1期,提示临床应用Iq7613时不宜与S期特异性药物(如甲氨喋呤、阿糖胞苷等)同时使用,以免产生自限作用而降低疗效,而应结合应用对G_1期敏感的药物(如博莱霉素、放线菌素等)或与放疗合用。
This study demonstrated that in vitro antiturnor agent Iq7613 could inhibit DNA synthesis of human cervical carcinoma HeLa cells directly; and the inhibitory ef-fect was more obvious with the increase of drug concentration and the prolongation of exposure time. Effect of Iq7613.on DNA in murine S180 ascitic cells was measured by computerized micrdspectrophotprneter. . The results showed that a large number of Siso ascitic cells accumulated in G1 phase and were completely retarded entering into S phase 4 hours after administration of Iq7613. Many GI phase cells were found until 24 hours after administration of Iq7613. It was suggested that Iq7613 is not suitable for Combination use with S phase specific agents (MTX, Ara C arid etc. ) Simultaneously, in order to avoid the lowering of therapeutic effect caused by self limiting effect Iq7613 should be combined with the agents sensitive to G1 phase cells (Bleomycin, Actinomycin and etc.), or with radiotherapy.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1993年第6期510-513,共4页
Chinese Journal of Cancer
