摘要
以甜菜丛根病根或病叶总RNA为模板,经反转录PCR扩增合成编码BNYVV CP的全长cDNA,将其克隆于pUCm-T载体上,获得了重组质粒pUCm-T/CP。经序列分析,此cDNA为一个567 bp长的开放阅读框架,编码由188个氨基酸组成的病毒外壳蛋白。又将CP基因构建到植物双元载体pROKⅡ中。最后通过三亲融合,得到了融合农杆菌LB4404。
Identification and characterization of a full-length cDNA encoding for BNYVV coat protein was described. The cDNA was 567 bp in length with open reading frame of 188 amino acids. The CP gene was inserted into the plant binary vector pROKII. And finally the recombinant vector pROKII was infused into Agrobac-terium tumefactions.
出处
《华北农学报》
CSCD
北大核心
2004年第2期37-39,共3页
Acta Agriculturae Boreali-Sinica
