摘要
报道了将马铃薯Ｙ 病毒（ＰＶＹ）中国分离株的外壳蛋白基因通过农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ ｔｕｍ ｅｆａ－ｃｉｅｎｓ）介导转入马铃薯（Ｓｏｌａｎｕｍ ｔｕｂｅｒｏｓｕｍ Ｌ．）的生产品种“Ｆａｖｏｒｉｔａ”、“虎头”和“克４”，在获得大量再生植株的基础上经过ＰＣＲ检测和Ｓｏｕｔｈｅｒｎ 杂交证明，大部分株系中ＰＶＹ 外壳蛋白基因的表达框架已完整整合到马铃薯的染色体上。人工接种ＰＶＹ 病毒（２０ ｍ ｇ／Ｌ）后一些转基因株系对ＰＶＹ 病毒的侵染表现较强的抗性，同时其单株结薯数和平均薯重有所增加。在田间实验中，转基因马铃薯植株生长良好而且部分转基因株系产量高于未转基因的脱毒马铃薯。
Potato virus Y(PVY) infection may cause a severe yield depression up to 80%.To develop the potato (Solanum tuberosum L.) cultivars that resist PVY infection is very crucial in potato production.The authors have been cloned the coat protein gene of PVY from its Chinese isolate.A chimaeric gene containing the cauliflower mosaic virus 35S promoter and PVY coat protein coding region was introduced into the potato cultivars “Favorita”,“Tiger head” and “K4” via Agrobacterium tumefaciens.Results from PCR and Southern blot analysis confirmed that the foreign gene has integrated into the potato chromosomes.These transgenic potato plants were mechanically inoculated with PVY virus (20 mg/L).The presence of the virus in the potato plants was determined by ELISA and method of back inoculation into tobacco.The authors observed a drastic reduction in the accumulation of virus in some transgenic potato lines.Furthermore,some transgenic potato lines produced more tubers per plant than the untransformed potato did,and the average weight of these transgenic plant tubers was also increased.In the field test,the morphology and development of these transgenic potato plants were normal,3 transgenic lines of “Favorita” exhibited a higher yield than the untrasformed virus free potato with an increase ranged from 20% to 30%.From these transgenic lines,it will be very hopeful to develop a potato cultivar which not only has a significant resistance to PVY infection,but also a good harvest in potato production.
基金
中国科学院"八五"攻关重大项目
香港王宽诚基金
