摘要
在水稻温敏失绿突变性状表达过程中,对其Rubsico 含量、Rubsico 活化酶活性,全叶蛋白及游离氨基酸组分变化进行测定。结果表明:突变体的Rubisco 结构和含量与野生型一样,保持相对稳定;而其Rubisco 活化酶活性则随一个分子量为56.2kD(PI=4.5)的特异蛋白质的存在与消失发生明显改变。当突变性状表达时,分子量为56.2kD(PT=4.5)的特异蛋白消失,其Rubisco 活化酶活性下降;当叶片失绿区域复绿时,56.2kD(PI=4.5)特异蛋白出现,则Rubisco 活化酶活性上升。这一密切地相关关系表明,突变体的Rubisco 活化酶活性变化在光合作用过程中,除与自身结构和含量有关外,还与叶片中这一特异蛋白的存在密切相关,它可能是Rubisco 活化酶活性的调节蛋白。这种调节具体表现在氨基酸代谢上,是对上游氨基酸的阻遏调控,从而使叶绿体的结构物质合成受阻,最终导致类囊体膜的退化。
We have determined the Rubisco components and activity,whole leaf protein andamino acid components of thermo-sensitive mutant line 1103s of rice (Oryza Sativa ssp.indica)leaves during induces green and yellow banding in this study.The results are as follow:Thestructure and components of Rubisco in the mutant are the same as in the wild form and rela-tively stable,but the activity of the mutant Rubisco greatly changes as a special protein ofmolecular weight 56.2kD (PI=4.5) appears and disappears.When the green-yellow bands appear,the special protein disappears,and the activity of the mutant-rice Rubisco decreases,whereaswhen the green-yellow bands in the same part of the leaves disappear,the special protein appearsand the activity of Rubisco is increase.The above shows the changes of the activity of the mu-tant-rice Rubisco during photosynthesis are closely related to the special protein in the leaves andits structure and components,and possibly to the regulating protein of Rubisco.The protein par-ticularly regulates metableic processes of amino acids preventing regulation of the preceding aminoacids,such that the formation of the structural material in chloroplast is prevented,and finally thechloroplast thylakoid structure degenerates.
出处
《实验生物学报》
CSCD
北大核心
2004年第3期183-188,共6页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金(39332353)
四川省教育厅重点项目经费资助
