摘要
研究了高水解度大豆多肽的制备和脱盐工艺,采用两种蛋白酶AS1.398和Alcalase水解大豆分离蛋白制得水解度为10%～24%的大豆多肽.结果显示:在等电点沉淀分离多肽时,相同水解度下,Alcalase酶水解产物的水解得率比AS1.398酶水解产物的水解得率高20%.采用DA201 C大孔吸附树脂对水解液进行脱盐,得到了优化的吸附和解吸的条件.该条件下大孔吸附树脂对水解液的吸附率为89.71%,解吸率为72.30%.
The experiment was conducted to investigate the technology involving in utilizing AS1.398 and Alcalase to hydrolyze SPI for preparing soybean polypeptides with DH of 10%～24%.The result shows that when separated peptides under isoelectric point precipitation,the yield of hydrolysates prepared by the action of Alcalase was 20% higher than that of AS1.398 at the same DH.The optimal condition for desalting with DA201-C macroporous resins was determined. Under the optimal condition,adsorption ratio was 89.71% and desorption ratio was 72.30%.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2004年第3期41-45,共5页
Journal of Wuxi University of Light Industry
关键词
大豆分离蛋白
酶法水解
脱盐
soybean protein isolates
enzymatic hydrolysis
desalting
