摘要
目的分析内源性端粒酶抑制基因PinX1与端粒酶、端粒相关蛋白在大肠癌中的表达 ,探讨PinX1在大肠癌演进过程中的作用及临床意义。方法应用半定量逆转录 聚合酶链反应(SQRT PCR)检测 6 6例大肠癌组织中内源性端粒酶抑制基因PinX1、端粒酶逆转录酶hTERT和端粒结合蛋白TRF1的mRNA表达水平。结果PinX1表达与大肠癌组织分化、临床分期和淋巴转移之间有显著相关性 (F分化 =11 4 0 ;F分期 =4 2 2 ;t=- 2 81,P≤ 0 0 5 ) ,与hTERT表达呈负相关 (r= - 0 5 5 3,P <0 0 1) ,与TRF1的表达无显著相关。PinX1高表达 (B =- 8 0 2 ,P <0 0 5 )和 (或 )不伴淋巴结转移 (B =- 1 6 9,P <0 0 5 )的患者预后较好。结论PinX1的下调可能与大肠癌形成、转移过程中端粒酶激活及维持机制有关 ,但对TRF1的调节还有赖于其他因素。检测PinX1的表达水平对评价大肠癌恶性程度、转移潜能和预后评估均有重要的临床价值。
Objective To study the role of endogenous telomerase catalytic inhibitor PinX1 in the progression of colorectal cancer tissue. Methods The expression of PinX1、telomerase subunits(hTERT) and telomere binding protein(hTRF1) was detected by semi-quantitative RT-PCR. Surgically resected colorectal cancer specimens in 66 cases were studied. Results PinX1 expression is significantly correlated with histologic differentiation,clinical stage and lymph node metastasis of colorectal cancer( Fd =11.40, Fs = 4.22,t =-2.81,P ≤0.05). The level of PinX1 was negatively associated with the expression of hTERT( r =-0.553,P <0.01 ),but not with hTRF1. Patients with higher PinX1 expression( B = -8.02 ,P <0.05) or/not combined with lymph node metastasis ( B =-1.69, P <0.05)have better prognosis. Conclusions Down-regulation of PinX1 may play an important role in the telomerase activation and maintenance in the development of colorectal cancer,but the regulation of hTRF1 may depend on other factors. The determination of PinX1 is important in evaluating the malignant degree,metastasis potency and predicting prognosis of colorectal cancer.
出处
《中华普通外科杂志》
CSCD
北大核心
2004年第3期157-159,共3页
Chinese Journal of General Surgery
基金
国家自然科学基金青年科学基金资助 (批准号 :3 0 3 0 0 415 )
