摘要
利用硝酸盐营养缺陷突变体 (nit)标记 ,对采自浙江、江苏、上海等地的 33个玉蜀黍赤霉菌株的营养亲和性进行研究。结果发现 ,这些菌株分别属于 30个不同的营养亲和群 (VCGs) ,表明中国玉蜀黍赤霉自然群体的遗传变异较大。用药剂驯化法获得了 6株ZF4 3菌株的多菌灵 (MBC)室内抗药突变体 ,并研究了其中 2株抗药突变体的营养亲和性能。结果表明 ,玉蜀黍赤霉对多菌灵产生抗药性后不会改变其营养亲和性能。但发现在营养亲和的两菌株之间发生菌丝融合以后 ,不能或很少能进行细胞核遗传物质的交换和重组 ,因此 ,菌丝融合在该菌对多菌灵的抗药群体发展中的作用较小。
Thirty-three isolates of Gibberella zeae collected from Zhejiang,Jiangsu and Shanghai belonged to 30 vegetative compatibility groups(VCGs)with nitrate-nonutilizing mutants(nit).It indicated that the highly degree of genetic diversity existed in natural population of G.zeae in China.Six laboratory carbendazim(MBC)-resistant mutants of ZF43 were obtained by fungicide-taming method,two of which were focused on the vegetative compatibility.The results revealed that the vegetative compatibility of G.zeae couldn′t change in the process of mutation for MBC-resistance.However,the nuclear gene couldn′t be exchanged or could exchange with very low chance between two vegetative compatible isolates after hyphal fusion.Therefore,hyphal fusion took very little effect on the development of MBC-resistant population in G.zeae.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2004年第2期39-42,共4页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目 ( 3 0 0 70 5 10 )
教育部高等学校骨干教师资助计划项目
