摘要
裙带菜的类囊体膜经过去污剂癸基-N-甲基匍萄糖胺增溶,采用SDS-PAGE分离技术,在Tris-Gly和Tris-硼酸两种电泳系统中分离其色素-蛋白质复合物,并比较其复合物的光谱特性。结果表明:采用Tris-Gly电泳分离系统从裙带菜中分离到8种色食-蛋白质复合物,分别是CP Ia、CPI、LHC_1、CPa、LHC_2、LHC_3、LHC_4和LHC_5。在Tris-硼酸电泳分离系统中共分离到5种色素-蛋白质复合物,分别是CPI、CPa、LHC_1、LHC_2、LHC_3。吸收光谱和荧光光谱的测定结果表明,两种电泳系统中分离的相对应条带的光谱特性基本相近。
When the thylakoid membranes of brown alga Undaria pinnatifida were solubilized by DMG and subjected to PAGE in
two electrophoresis systems, there were eight kinds of pigment-protein complexes separated in Tris-Gly system, and they were
CPⅠ a、CPⅠ、LHC_1、CPa、LHC_2、LHC_3、LHC_4、LHC_5. In Tris-Boric acid system, five kinds of pigment-protein complexes were
isolated, and they were CPⅠ、CPa、LHC_1、LHC_2、LHC_3. The absorption and fluorescence emission spectra were measured and
compared. The results show that the characteristics of the sane kind of pigment-protein complexes resolved from different
electrophoresis systems are similar.
出处
《生态科学》
CSCD
2004年第1期32-34,共3页
Ecological Science
基金
山东省科学基金(Y99D_2)
关键词
裙带菜
色素-蛋白质复合物
电泳
吸收光谱
荧光发射光谱
Undaria pinnatifida
Pigment-protein complexes
PAGE
Absorption spectra
Fluorescence emission spectra
