摘要
近年来,大量采用体外细胞培养技术研究垂体细胞分泌功能。原代培养垂体细胞通常采用添加血清或组织抽提物的培养液,血清通过提供营养物、激素、载体蛋白和各种生长调节因子促进细胞新陈代谢,尤其对垂体细胞的发育和分化、受体的诱导及表达、激素或神经递质的产生均具有重要作用.但血清成分复杂,其中存在的未知因子可能使实验结果混乱。本实验采用无血清原代培养大鼠垂体前叶和中/后叶细胞,细胞收获量大,形态完整,分泌功能正常。
A method for primary culturing of rat anterior and intermediate/posterior pituitary cells in couplete serum-free defined medium (CSFM) is described. Dispersed pituitary cells were prepared by using a multiple enzyme digestion system. Insulin, transferrin and serum albumin were essential additives for maintenance of primary rat pituitary cells in CSFM. Morphological immunocy-tochemical and RIA studies during three weeks' culture indicated that β-endorp-hin-like immunoreactivity (β-End-IR) was contained in and released from pituitary cells.The secretion rate remained almost constant for 2 weeks after the 5th day in culture. This method provides an ideal in vitro model for studying the effects of various factors on the synthesis and release of β-endorphin in pituitary cells and the mechanism. of regulation of other target gland hormones.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第3期225-228,T009,共5页
Acta Academiae Medicinae Sinicae
