摘要
目的 :探讨NF -κB“decoy”寡核苷酸 (ODNs)对脂多糖 (LPS)诱导小鼠巨噬细胞株J774 1产生一氧化氮 (NO)及活性氧的影响。方法 :通过体外细胞培养技术 ,观察转染NF -κB“decoy”ODNs对LPS刺激巨噬细胞产生NO和活性氧 (ROS)的影响 ,以及诱导型一氧化氮合酶 (iNOS)活力的变化。结果 :在LPS刺激的巨噬细胞中转染NF -κB“decoy”ODNs减少NO和ROS的产生 ,降低iNOS的活力。结论 :NF -κB“decoy”ODNs可以对抗LPS诱导巨噬细胞产生的ROS和NO ,可能与其特异性竞争抑制活化NF -κB结合位点有关。
AIM: To study the effects of nuclear factor-kappa B decoy oligodeoxynucleotides on generation of nitric oxide and activated oxygen in lipopolysaccharide-induced mouse macrophages.METHODS: Mouse macrophage cell lines J774.1 cells were cultured with lipopolysaccharide and liposome-mediated oligodeoxynucleotides, and the level of nitric oxide, activated oxygen and inducible nitric oxide synthase were measured. RESULTS: Nuclear factor-kappa B “decoy”oligodeoxynucleotides decreased the activity of inducible nitric oxide synthase in lipopolysaccharide-induced macrophage, inhibited generation of nitric oxide and reactive oxygen species. The level of nitric oxide, activated oxygen and inducible nitric oxide synthase did not change in control group.CONCLUSION: Nuclear factor-kappa B “decoy”oligodeoxynucleotides decrease the generation of nitric oxide and activated oxygen in lipopolysaccharide-induced macrophages, which is probably due to inhibiting the binding sites of activated nuclear factor-kappa B specially.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第5期757-759,共3页
Chinese Journal of Pathophysiology
