摘要
应用PCR-SSO方法对360例正常人(8例中国人,余为日本人)HLA-DR型别进行了检测,结果表明:使用FPR_1和DRβAMP_1引物,可特异地扩增编码HLA-DR分子β_1区的DRB基因第2外显子的部分DNA片段,再根据所扩增片段中各型HLA-DR间碱基的特异性,合成18～20mer的SSO,并用SSO与扩增的HLA-DR DNA片段进行杂交,基本上可确定目前已知的各型HLA-DR。
PCR-SSO method was used to detect the genotyping of HLA-DR in 360 normal persons (8 Chinese and 352 Japanese). Results indicated that using FPR_1 and DRβAMP_1 primers the DNA fragment coding the HLA-DRβ_1 region could be specifically amplified. The amplified DNA fragment contains the second and third super-variable regions of second exon of DRB genes. According to the base specifcity in the second and third super-variable regions of individual HLA-DR, the sequence specific oligonucleotides (SSOs)with 18-20 mer were synthesized and used to hybridize with the DNA fragment of HLA-DR amplified by PCR method, by which the known HLA-DR can be genotyped. This method is highly sensitive and specific.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1992年第4期193-195,T013,共4页
Chinese Journal of Medical Genetics
关键词
人白细胞抗原
多态现象
寡核苷酸类
HLA-DR antigens Poly morphism (Genetic) Polymerase chain reaction Oligonucleotide
