摘要
目的:探讨CDC25B(cell division cycle 25B,细胞分裂周期蛋白25B)在大肠肿瘤中的表达及临床意义. 方法:应用半定量的RT-PCR及免疫组化染色(S-P法)方法检测该基因在大肠癌(RT-PCR法,n=24;S-P法,n=168), 大肠腺瘤(RT-PCR法,n=62;S-P法,n=25)及大肠正常黏膜(RT-PCR法,n=10;S-P法,n=20)中的表达. 结果:CDC25B mRNA在大肠癌及大肠腺瘤中的相对含量明显高于正常大肠黏膜(1.41±0.07,1.32±0.17 vs 0.62±0.02,P<0.01),在大肠腺瘤伴轻-中-重度不典型增生组织中的相对含量逐渐增高(1.25±0.21,1.36±0.19和1.40±0.07,P<0.01);CDC25B基因翻译蛋白在正常大肠黏膜、腺瘤中未见表达,在大肠癌中以细胞核表达为主, 其表达与大肠癌有无远处脏器转移相关(2/46 vs 14/106, P<0.05);经随访发现CDC25B蛋白阳性表达者,5 a生存率明显降低(25/31 VS 42/82,P<0.05). 结论:CDC25B可能参与了大肠腺瘤到大肠癌的转变过程, 其在大肠癌发展中可能起一定作用,为大肠癌预后不良的危险因素.
AIM: To elucidate the role of CDC25B in pathogenesis, evolution and metastasis of colorectal cancer and to investigate the relationship between the expression of CDC25B and the clinical pathological features of colorectal cancer and the prognosis of patients. METHODS: The expression of CDC25B at the mRNA and protein levels was examined in patients with colorectal cancer (n=24 by RT-PCR methods and n=168 by S-P methods), colorectal adenoma (n=62 by RT-PCR methods and n=25 by S-P methods) and normal colorectal mucosa (n=10 by RT-PCR methods and n=20 by S-P methods) by RT-PCR and immunohistochemical S-P method. The survival of postoprative patients were followed-up. RESULTS: The CDC25B mRNA expression in colorectal cancer and colorectal adenoma was higher than that of nomal colorectal tissues (1.41±0.07, 1.32±0.17 vs 0.62±0.02, P<0.01), and the CDC25B mRNA of colorectal cancer was higher than that of colorectal adenoma (1.41±0.07 vs 1.32±0.17, P<0.05). The expression of CDC25B mRNA in colorectal adenoma with mild, moderate to severe hyper-plasia was gradually increasing (1.25±0.21 vs 1.36±0.19 vs 1.40±0.07, P<0.01). The expression of CDC25B had a positive correlation with distant metastases. The patients whose expression of CDC25B was positive had a markedly low survival rate in 5 years. CONCLUSION: CDC25B may be involved in the transition from adenoma to cancer. Expression of CDC25B in colorectal cancer may accelerate the transformation of cell cycle, which promotes the metastases to distant organs.
出处
《世界华人消化杂志》
CAS
2004年第5期1057-1060,共4页
World Chinese Journal of Digestology
