摘要
目的 研究人类细胞色素P4 5 0 (cytochromeP 4 5 0 ,CYP 4 5 0 ) 2A(CYP2A)在鼻黏膜中的表达特点和意义。方法 应用免疫印迹方法分别检测 36例鼻腔鼻窦疾病患者和孕 96、119、16 0、182、185d的 5例胎儿不同部位鼻组织中CYP2A的表达水平 ,并用免疫组化方法检测CYP2A在嗅区黏膜中分布特点。结果 成人标本中 10块嗅区黏膜标本中 8例免疫印迹阳性 ,而 37块呼吸区黏膜标本均为阴性。胎儿嗅区黏膜标本免疫印迹均为阳性 ,其表达水平随胎龄有增加的趋势 ,仅 1例呼吸区黏膜检测到CYP2A。免疫印迹定量分析显示 ,成人嗅区鼻黏膜微粒体CYP2A蛋白质水平在0 1～ 1 1pmol/mg之间 ,胎儿的鼻微粒体蛋白质含量在 0 8～ 5 3pmol/mg之间 ,胎儿一般高于成人。免疫组化结果显示 ,CYP2A免疫活性存在于嗅黏膜的支持细胞和Bowman腺。结论 CYP2A存在于嗅黏膜提示 :嗅黏膜是吸入性外源性物质生物转化的靶位 ,CYP2A还与嗅觉感受有关 。
Objective To investigate the nasal expression of CYP2A proteins in human Methods Immunoblot analysis was used to detect fetal tissues at 96 185 days of gestational age and in surgical biopsy tissues from different regions of nasal cavity of 36 adult patients, and immunohistochemical methods were used to localize CYP2A proteins′ cellular distribution Results In adults, CYP2A protein was detected in olfactory microsomes from 8 of 10 individual, but not in the respiratory nasal microsomes from 37 individual Quantitative immunoblot analysis confirmed that CYP2A proteins were selectively expressed in the olfactory region in both adult and fetal tissues Interestingly, the levels of CYP2A proteins in nasal microsomes were generally higher in fetuses than in adults The CYP2A protein content in olfactory microsomes ranged from 0 1 to 1 1 pmol/mg among adult nasal microsomes, while among five fetuses ranged from 0 8 to 5 3 pmol/mg microsomal protein, with a trend of developmental increase in expression level Immunohistochemical studies confirmed that the CYP2A proteins were expressed in the supporting cells in the olfactory epithelium and in the Bowman′s glands in the lamina propria Conclusions CYP2A proteins expression in human olfactory mucosa indicates that olfactory mucosa is metabolic target site of the xenobiotics Also CYP2A has a relationshipe with olfaction The prenatal expression of the CYP2A proteins in the OM suggests potential risks of developmental toxicity from maternally derived xenobiotics
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2004年第2期105-107,共3页
Chinese Journal of Otorhinolaryngology
基金
国家自然科学基金资助项目 ( 3 95 70 760 )
