摘要
目的 观察α骨化醇对肾辐射损伤骨质疏松大鼠骨质量的保护作用。方法 6月龄健康雄性SD大鼠 30只 ,随机分为模型 (Ⅱ ) ,假手术 (Ⅰ )和α骨化醇治疗 (Ⅲ ) 3组 ,每组 10只。以 15Gy1 37Csγ射线照射大鼠肾脏局部制备肾辐射损伤模型 ,照射后 1周 ,α骨化醇治疗组给予萌格旺 0 1μg·kg体重 - 1 ·d- 1 ,以 1%羧甲基纤维素钠 (CMC)制成混悬液灌胃 ,1ml/只 ,模型组以 1%CMC灌胃 ,连续 3个月。实验结束后 ,各组大鼠取L1 ～L4 腰椎及右侧股骨 ,分别测定骨密度 (BMD)、骨形态计量和生物力学等指标。结果 与Ⅰ组比较 ,Ⅱ组的腰椎骨密度明显降低 ,骨小梁体积、厚度减少 ,骨小梁间距增宽 ,矿化沉积率 ,股骨、腰椎的最大载荷明显降低 (P <0 0 5 ,P <0 0 1)。α骨化醇治疗组与模型组比较 ,腰椎骨密度明显提高 (P <0 0 1) ,骨小梁体积、宽度显著增加 ,骨小梁间距变小 (P <0 0 5 ,P <0 0 1) ,矿化沉积率 ,股骨、腰椎的最大载荷明显提高 (P <0 0 5 ,P <0 0 1)。结论 α骨化醇对肾局部辐射损伤导致骨质疏松大鼠骨质量有明显改善作用。
Objective To study the protective effect of alpha-calciferol on bone metabolism in rats with radiation injury to kidney. Methods Six-month-old male SD rats were randomly divided into three groups:model group,sham group,and alpha-calciferol (Bone-One) group,10 rats each.The model group was developed by irradiation of rat kidneys with 15 Gy of Cs 137 γ-rays.One week after radiation,alpha-calciferol group was given orally Bone-One,0.1 μg·kgBW -1·d -1,suspended in 1% CMC,1 ml a rat for 90 days.BMD of L_ 1-4,bone histomorphometry,bone biomechanical properties were measured in all rats at the end of experiment. Results BMD of L_ 1-4 was significantly lowered,volume and thickness of trabecular bone were significantly decreased,space of trabecular bone increased,mineral apposition rate (MAR),maximal load of femur and lumbar vertebra decreased ( P <0.05, P <0.01) in model group,compared with sham group.In alpha-calciferol group,BMD of L_ 1-4,volume and thickness of trabecular bone increased,space of trabecular bone decreased significantly ( P <0.05, P <0.01),MAR,maximal load of femur and lumbar vertebra increased significantly ( P <0.05, P <0.01),compared with model group. Conclusion Alpha-calciferol improves disturbance of bone metabolism in rats with radiation injury to kidney.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2004年第2期125-127,共3页
Chinese Journal of Radiological Medicine and Protection
