摘要
目的 :探讨急性炎症状态下发生心肌缺血时对心脏的影响 .方法 :2 4只成年家兔 (1 .8～ 2 .5kg)随机分为空白对照组 (A) ,单纯心肌缺血组 (B) ,内毒素组 (C) ,内毒素加心肌缺血组 (D) ,建立AMI模型 ,术后 6h观察血液学指标及缺血心肌的HE染色、COX 2多克隆抗体免疫组织化学染色 .结果 :血液学指标示术后 6hMDA ,NO ,髓过氧化物酶 (MPO)测定值D组与A ,B ,C组有明显差异 (P <0 .0 5 ) ;后 3组心房钠尿肽释放增加 ,D组与B ,C组有明显差异 (P <0 .0 5 ) ,D组左室舒张末压、左室收缩峰压测定值较B ,C组降低明显 ,缺血心肌中环氧化酶 2高度表达 .结论 :机体处于炎症状态时发生心肌缺血 ,炎症因子损害心肌 。
AIM: To investigate the effects of experimental acute myocardial infarction (AMI) on rabbits with systemic inflammation. METHODS: Adult rabbits (weight: 1.8-2.5 kg; n =24) were randomly divided into 4 groups: group A: normal controls; group B: myocardial ischemia: The left circumflex arteries of rabbits were ligated to establish the animal models of AMI; group C: Lipopolysaccharide (LPS) and group D: myocardial ischemia + LPS. Six hours after AMI, the serum MDA, NO, MPO and ANP were measured and myocardial HE and COX 2 expression were determined by immunohistochemical staining. RESULTS: Plasma MDA, NO and MPO increased significantly 6 h after AMI in groups B, C and D, and those in group D increased more significantly compared with those in groups B and C( P < 0.05) . ANP and LVEDP and LVPEP significantly increased in group D compared with those of group B( P <0.05). In group D, the myocardial COX 2 expression was significantly higher than that in group B and C. CONCLUSION: Inflammatory factors damage the myocardium and aggravate the heart function.
出处
《第四军医大学学报》
北大核心
2004年第7期645-647,共3页
Journal of the Fourth Military Medical University
