摘要
目的 :揭示乙肝病毒 (hepatitisBvirus,HBV)感染胎盘滋养层细胞的可能方式 ,探讨乙肝表面抗原 /膜联蛋白V(HBsAg/AnnexinV)检测在HBV宫内传播机制研究的意义 .方法 :收集血清学HBsAg阳性孕妇足月胎盘组织 39例 ,经SABC免疫组化方法在其中 2 3例中检测到HBsAg ,随机选取 7例采用免疫荧光双标记方法结合激光共聚焦技术检测HBsAg/An nexinV .结果 :血清学HBsAg阳性孕妇足月胎盘组织中HB sAg/AnnexinV共存于滋养层细胞胞质中 .结论 :提示在HBV宫内传播机制方面存在着这样一种可能 ,即AnnexinV结合并携带HBV进入滋养层细胞 ,从而使滋养层细胞受到HBV感染 .
AIM: To find out the possible form Hepatitis B Virus takes in the infection of trophblasts and to study the role of HBsAg/Annexin V in the HBV intrauterine transmission. METHODS: Thirty nine full term placentae of HBsAg sero positive pregnant women were collected, in which 23 were detected as HBsAg positive by SABC immunohistochemistry staining. Seven out of the 23 were selected randomly for the detection of HBsAg/Annexin V. The immunofluorenscence assay and confocal laser scanning microscope were conducted. RESULTS: HBsAg/Annexin V co existed in the cytoplasm of trophoblasts in the full term placentae of HBsAg sero positive pregnant women. CONCLUSION: It is suggested that Annexin V, as a receptor, carries HBV into the trophoblasts, resulting in the trophoblast infection.
出处
《第四军医大学学报》
北大核心
2004年第7期581-583,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金 (30 2 30 32 0 )
