摘要
本文以青霉素G.K为例,用汞膜电极研究了在铜(Ⅱ)存在下含β-内酰胺环抗菌素的吸附溶出伏安行为,认为其电极过程为先化学反应而后吸附富集再进行电化学溶出的过程.同时,还对这类抗菌素的水解条件及降解产物的稳定性作了研究,确定了最适条件.在柠檬酸—磷酸二氢钠(pH2-7)介质中,青霉素的水解产物青霉素酸的浓度在1.0×10^(-9)～5.0×10^(-6)mol.L^(-1)范围内与溶出峰电流均有良好的线性,富集10min,检测限低至1.0×10^(-10)mol.L^(-1).
The adsorptive stripping behaviour of B-lactanring antibiotics such as penicillin G.K. on mercury-film electrode in the presence of copper (Ⅱ) has been studied and the mechanism is discussed.The process of accumulation is considered to be an adsorptive process behind a chemical reaction. Besides the condition of hydrolysis and the slability of degradation products have been discussed and the optimum has been decided. In H3C6H5O7-Na2HPO4 (pH2.7) solution, the concentration of hydrolytic product, penicillin-BPA over range of 1.0×10-9～5.0×10-6 mol·L-1 is in good linear retationship with the stripping peak current values, and the detection limit after 10 min accumulation is as low as 1.0×10-10mol·L-1
关键词
内酰胺环抗菌素
铜
吸附溶出伏安法
抗菌素
β-laetan ring anlibiotics
Adsorptive voltammetric
Trace analysis
