摘要
目的 研究热休克蛋白 70 (HSP 70 )在高温处理后大鼠肝中不同时段的表达。方法 SD大鼠随机分成A组 (对照组 )和B组 (高温组 ,42℃ ,2 0min)。A组分别在麻醉后 4,8,2 0 ,3 6,72h取肝脏组织 ;B组置于自行设计的加热装置分别热处理后 4,8,2 4,3 6,72h取肝脏组织。用免疫组织化学、原位杂交方法检测A ,B两组不同时段HSP70表达 ,并对比其差异。结果 高温处理组各时段HSP70的表达差异有显著性 (P <0 .0 5 ) ;8～ 2 4h达最高峰 (P <0 .0 1)。高温处理后HSP70的表达明显高于对照组 (P <0 .0 5 )。结论 高温可诱导HSP70在大鼠肝中的高表达 ,处理后不同时段表达的量不同。
Objective To investigate the expression of hyperthermia-induced HSP70 in liver during the different period after heating. Methods SD rats were divided randomly into tow groups:Group A (room control group),rats′ liver tissues were removed at the 4 h,8 h,24 h,36 h and 72 h after anaesthesia respectively.Group B(hyperthermia group) rats were heated up by self-designed heating device (42℃,20 min),and then those liver tissues were harvested at the different time respectively as in the group A. Results HSP70 expression in livers of group B were significantly higher than that in group A(P<0.05). Hyperthermia resulted in the highest expression of HSP70 from 8 h to 24 h after heating (P<0.01) of the liver in rats. Conclusions The expression of HSP70 can be induced by body hyperthermia and the expression is different at different time after heating.
出处
《中国普通外科杂志》
CAS
CSCD
2004年第6期432-434,共3页
China Journal of General Surgery
