摘要
目的建立紫菀止嗽颗粒中紫菀酮的HPLC测定方法。方法采用phenomenexC18柱(250×4.6mm)为固定相;乙腈为流动相;流速1mL/min;检测波长203nm。结果紫菀酮在0.020～0.100μg/μL范围内线性关系良好,r=0.9996;平均加样回收率为97.8%,RSD=2.37%(n=5)。结论该研究建立的紫菀酮含量测定方法灵敏、准确、简单易行、重现性好。
Objective To establish a HPLC method for the determination of Shionone in Ziwan Zhisou granule. Methods Phenomenex C18 column (250× 4.6 mm) was used. The mobile phase was acetonitrile and the detection wave length was at 203 nm. Result A good linearity of Shionone was in the range of 0.025～ 0.250 μ g/μ L, r=0.9996. The average recovery was 97.8 % , RSD was 2.37 % ( n=5) . Conclusion This method is sensitive, accurate and simple and with good reproducibility for the determination of Shionone in Ziwan Zhisou granule.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第3期197-199,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
