摘要
目的 :测定正常大鼠气道平滑肌细胞 (ASMCs)中Ryanodine受体 (RyanodinereceptorRyR)各亚型mRNA的表达以及在哮喘中的变化。方法 :采用胶原酶消化法培养大鼠ASMCs ,RT PCR测定受体各亚型mRNA的表达。卵蛋白致敏并激发大鼠制备慢性哮喘模型 ,RT PCR测定各受体mRNA表达水平的变化。结果 :大鼠ASMCs中可见RyR1、RyR2和RyR3三种RyR亚型mRNA共表达 ,扩增片段测序结果与GenBank中登录的大鼠RyR1- 3序列完全一致。制备的慢性哮喘模型中 ,病理切片显示平滑肌层及黏膜层明显增厚 ,RyR1的表达较对照组有明显的上调P <0 .0 5。结论 :大鼠气道平滑肌细胞中RyR三种亚型共表达 ,提示存在着复杂的细胞内钙调节机制 ;并且RyR1在慢性哮喘的发生过程中可能起了一定的作用。
Aim: To detect the expression of ryanodine receptor(RyR) subtypes in normol rat airway smooth muscle cells(ASMCs) and changes during chronic asthma formation. Methods: ASMCs were cultured with collagen enzyme digestion method. The expression of subtyes of RyR were detected by RT-PCR. Purified PCR product linked with pGEM-T vector to make DNA sequence assay. Chronic asthma model was made with OVA, the changes of RyRs detected by RT-PCR. Results: All subtypes of RyR were expressed in airway smooth muscle cells of normol rat. The expression of RyR1 increased obviously compared with control group(P<0.05) on chronic asthma.Conclusion: Co-expression of three subtypes of RyR in ASMCs of normal rat, indicate that there are complicated intercellular Ca 2+ regulation mechanism in ASM, moreover RyR1 might play a role during asthma development.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2004年第2期181-184,共4页
Chinese Journal of Applied Physiology
基金
军队"十五"攻关资助课题 ( 0 1MA0 68)
