摘要
目的 研究精氨酸升压素 (AVP)对大鼠心肌成纤维细胞诱导型一氧化氮合酶 (iNOS) 一氧化氮 (NO)系统活性的影响。方法 胰酶消化法分离培养Sprague Dawley仔鼠心肌成纤维细胞 ,采用硝酸还原酶法、蛋白质印迹和逆转录 聚合酶链式反应观察AVP对心肌成纤维细胞的NO含量、iNOS蛋白水平和iNOSmRNA表达的影响。结果 (1)不同浓度AVP干预下 ,心肌成纤维细胞的NO含量、iNOS蛋白水平和iNOSmRNA表达都随AVP浓度的增高而增加。其中 10 -7mol/LAVP组和 10 -6mol/LAVP组的NO含量 [(6 9 0 5± 5 5 6 ) μmol/L和 (6 2 86± 6 0 5 ) μmol/L]、iNOS蛋白水平 (0 73± 0 0 6和 0 6 4± 0 0 5 )和iNOSmRNA表达 (0 70± 0 0 3和 0 6 6± 0 0 6 )都显著高于对照组 [(2 9 34± 5 34)μmol/L ,0 2 0± 0 0 5 ,0 2 5± 0 0 4 ]、10 -9mol/LAVP组 [(31 79± 6 5 9) μmol/L ,0 2 4± 0 0 7,0 30±0 0 6 ]和 10 -8mol/LAVP组 [(36 87± 7 89) μmol/L ,0 30± 0 0 9,0 31± 0 0 3]。但 10 -6mol/LAVP组的NO含量、iNOS蛋白水平和iNOSmRNA表达都低于 10 -7mol/LAVP组。 (2 ) 10 -7mol/LAVP干预下CFs的NO含量、iNOS蛋白水平和iNOSmRNA表达都随培养时间的延长而增加。其中 2 4h组和 36h组的NO含量 [(6
Objective To explore the effects of arginine vasopressin (AVP) on inducible nitric oxide synthase (iNOS) nitric oxide (NO) system activity in cultured rat cardiac fibroblasts. Methods Cardiac fibroblasts were isolated by trypsin digestion method. Nitric acid reductase method, Western blotting and reverse transcription polymerase chain reaction were used to detect NO contents, iNOS protein synthesis and iNOS mRNA expression respectively. Results (1) AVP increased NO contents, iNOS protein synthesis and iNOS mRNA expressions in a concentration dependent manner. Moreover, NO contents [(69 05±5 56) μmol/L and (62 86±6 05) μmol/L], iNOS protein synthesis (0 73±0 06 and 0 64±0 05) and iNOS mRNA expressions (0 70±0 03 and 0 66±0 06) of 10 -7 mol/L AVP group and 10 -6 mol/L AVP group were both higher than those of control group (29 34±5 34 μmol/L, 0 20±0 05, 0 25±0 04), 10 -9 mol/L AVP group [(31 79±6 59) μmol/L, 0 24±0 07, 0 30±0 06] and 10 -8 mol/L AVP group [(36 87±7 89) μmol/L, 0 30±0 09, 0 31±0 03]. But NO contents, iNOS protein synthesis and iNOS mRNA expressions of 10 -6 mol/L AVP group were both lower than those of 10 -7 mol/L group. (2) 10 -7 mol/L AVP increased NO contents, iNOS protein synthesis and iNOS mRNA expressions in a time dependent manner. Moreover, NO contents [(65 05±5 56) μmol/L and (62 43±6 21)μmol/L], iNOS protein synthesis (0 69±0 07 and 0 65±0 05) and iNOS mRNA expressions (0 69±0 10 and 0 68±0 05) of 36h group and 24 h group were both higher than those of 6 h group [(33 92±4 05) μmol/L, 0 26±0 05, 0 18±0 05] and 12 h group [(45 11±4 33) μmol/L, 0 34±0 04, 0 24±0 05] But NO contents, iNOS protein synthesis and iNOS mRNA expressions of 36 h group were both lower than those of 24h group Conclusion AVP stimulates iNOS NO system activity in cultured cardiac fibroblasts, the enhancement of NO contents may be able to inhibit ventricular remodeling induced by AVP.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2004年第3期245-249,共5页
Chinese Journal of Cardiology
