摘要
目的 探讨心房颤动 (房颤 )心房组织内细胞外信号调节激酶 (ERK1、ERK2 )和血管紧张素转换酶 (ACE)表达的变化。方法 5 2例风湿性心脏病患者 ,在心脏外科手术时取右心耳组织 ,采用逆转录 聚合酶链反应 (RT PCR)技术测定ERK的mRNA表达量 ,免疫印迹 (Westernblotting)测定磷酸化ERK1、ERK2 ,ERK激活激酶 (MEK1、2 )和ACE蛋白水平 ,免疫组织化学技术观察磷酸化ERK1、ERK2在心房组织中的分布。结果 持续性房颤组 (19例 )ERK2 mRNA表达量以及磷酸化ERK1、ERK2 、ACE和MEK1、2 量较窦性心律组 (2 1例 )均明显增加 [ERK2 mRNA :(76 1± 19 7)U与 (30 9± 2 4 0 )U ,P <0 0 5 ;ERK1:(2 4 8± 5 4 ) %与 (10 0± 2 1) % ,P <0 0 1;ERK2 :(30 2± 4 9) %与 (10 0± 2 2 ) % ,P <0 0 1;ACE :(2 98± 4 5 ) %与 (10 0± 2 4 ) % ,P <0 0 1;MEK1、2 :(16 9± 2 1) %与 (10 0± 8) % ,P <0 0 5 ];持续性房颤组和阵发性房颤组 (12例 )之间各指标均无明显差别 ;免疫组织化学显示增加的ERK1、ERK2 均分布在心房间质细胞。结论 研究提示心房间质细胞内激活的磷酸化ERK1、ERK2 表达的增加可能有赖于心房组织ACE表达的增加 ,这可能是房颤时心房纤维化的分子机制之一。
Objective To investigate whether atrial expression of the extracellular signal regulated kinase (ERK 1/ERK 2) and of the angiotensin converting enzyme(ACE) is altered in patients with atrial fibrillation(AF). Methods A total of 52 patients with rheumatic heart disease were examined. Atrial tissue was obtained from the right appendage during open heart surgery. ERK expression was analyzed at mRNA level by reverse transcription polymerase chain reaction(RT PCR),at the protein level by Western blotting and at artial tissue level by immunohistochemistry. ERK activating kinase(MEK 1、2 ) and ACE were analyzed by Western blotting techniques. Results Increased amounts of ERK 2 mRNA were found in patients with chronic AF[ (76.1±19 7)U vs sinus rhythm: (30 9±24 0)U; P <0 05]. Activated ERK 1、ERK 2 and MEK 1、2 were significantly increased in patients with AF compared to patients with sinus rhythm. No difference between chronic AF and paroxymal AF was found. The expression of ACE was three fold increased in patients with chronic AF compared to patients with sinus rhythm. Patients with AF showed an increased expression of ERK 1/ERK 2 in atrial interstitial cells and marked fibrotic tissues. Conclusions An atrial tissue ACE dependent increase in the amounts of activated ERK 1/ERK 2 in atrial interstitial cells may be a molecular mechanism for the development of atrial fibrosis in patients with AF . These findings may have an important impact on the treatment of AF.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2004年第3期207-210,共4页
Chinese Journal of Cardiology
基金
华中科技大学同济医学院附属协和医院科研基金资助
