摘要
目的 用Wistar大鼠研究瘦素 (leptin)在颗粒脂肪移植中的促进血管增生作用。方法 取大鼠腹部脂肪 ,分别经 2 0 μg/L瘦素和生理盐水处理后 ,以颗粒脂肪的形式自体移植。移植组织在不同观察时间取出后称重 ,同时行碱性磷酸酶 (AKP)染色及α 肌动蛋白 (Actin)染色 ,用计算机图像分析技术测定血管密度。实验结果用SPSS统计分析软件进行统计学分析。结果 瘦素处理组血管面密度在移植 10d、2 0d后分别为 (0 .12± 0 .0 4 ) μm2 / μm2 、(6 .83± 1.86 )× 10 -2 μm2 / μm2 ,显著高于同一观察时间的生理盐水处理组 (P <0 .0 5 )。Actin染色强度也较高。结论 一定浓度的瘦素对移植鼠颗粒脂肪组织具有促进血管增生作用。
Objective To investigate the angiogenic effect of leptin on grainy fat transplantation. Methods Grainy fat was obtained from the Wistar rat subcutaneous tissue in the abdomen. It was then mixed with 0.9 % NS and 20 ng/ml leptin and transplanted in the head subcutaneously. The transplanted tissue was taken out after 10, 20 and 40 days and was stained with AKP histochemistry and actin immunohistochemistry. The microvessel density was measured by medical imagine analysis system. The data were analyzed with SPSS software. Results The values of microvessel density in leptin groups were 0.12 ±0.04μm 2/μm 2 after 10 days and (6.83±1.86)×10 -2 μm 2/μm 2 after 20 days. This was obviously high as compared with (1.49±0.88)×10 -2 μm 2/μm 2 and (4.32±2.11)×10 -2 μm 2/μm 2 in saline groups in the same observing periods ( P <0.05). Actin staining in leptin groups was also intense. Conclusion Leptin may be a candidate angiogenesis factor in grainy fat transplantation.
出处
《中华医学美学美容杂志》
2004年第1期34-36,共3页
Chinese Journal of Medical Aesthetics and Cosmetology
