摘要
目的 建立一种快速、简便、高灵敏测定血清铁的新方法。方法 在非离子型表面活性剂聚氧乙烯月桂醚 (Brij 35 )及聚氧乙烯异辛基苯基醚 (TritonX 10 0 )存在下 ,用水溶性试剂 2 (5 硝基 2 吡啶偶氮 ) 5 [N 正丙基 N (3 磺酸丙基 )氨基 ]苯酚 (简称Nitro PAPS)作显色剂直接光度法测定血清铁。结果 该方法测定血清铁 ,显色络合物最大吸收波长为 5 90nm ,线性范围达 10 7 4 μmol/L ,表观摩尔吸光系数为 1 0 3× 10 5L/(mol/cm)。回收率为 97 9%～ 10 2 2 % ,批内变异系数 (CV)为2 4 %～ 3 5 % ,批间变异系数 (CV)为 2 9%～ 3 7% ,与亚铁嗪分光光度法比较相关良好 ,Y =0 992X +0 12 ,r=0 9883,P >0 0 5。 85名健康成年人血清铁含量为 (11 2～ 2 8 4 ) μmol/L ( x±2s)。结论 该法血清用量少 ,不必去蛋白 ,具有操作快速、简便、结果灵敏可靠等优点 。
Objective To establish a simple sensitive and stable new method of etermination of iron in serum by UV Spectrophotometry Methods 2 (5 nitro 2 pyridylazo) 5 (N propyl N sulfopropylamino)phenol was used as a coloring reagent to determine iron by spectrophotometry in the presence of surfactants Brij 35 and Triton X 100 Results The maximum absorption of the complex compound was at 590 nm, The linearity of test was up to107 4 μmol/L. The molar absorption coefficient is 1 03×10 5L/(mol/cm) The recoveries ranged from 97 9% to 102 2% The within run and between run CV s are 2 4%～3 5% and 2 9%～3 7% Comparing this method ( Y ) with Ferrospectral method, we obtained the regression equation Y =0 992 X + 0 12, r =0 988 3, P >0 05, the reference interval ( ±2s ) was (11 2～28 4) μmol/L( n =32). Conclusions This method is simple Sensitive, rapid and accurate,and suitable for the routine manual or automatic analysis of iron in serum suitable for clinical application
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2004年第3期137-139,共3页
Chinese Journal of Laboratory Medicine
关键词
高灵敏分光光度法
血清铁
测定
血清学试验
Serologic tests
Spectrophotometry
2 (5 nitro 2 pyridylazo) 5 (N propyl N sulfopropylamino)phenol
