摘要
目的 观察星状神经节阻滞(SGB)对全脑缺血再灌注损伤家兔海马及颞叶皮质热休克蛋白70(HSP70)表达的影响,探讨SGB对全脑缺血再灌注损伤的处理效应及可能机制。方法 健康日本大耳白兔28只,随机分成四组(n=7):SGB组(E组)、生理盐水对照组(P组)、空白对照组(C组)和假手术组(S组)。采用星状神经节旁置管法制作SGB模型及六血管阻断法制作全脑缺血再灌注损伤模型。缺血10 min再灌注30 h(E、P、C组)或观察相应时间(S组)后取脑组织,使用免疫组化技术检测双侧海马CAl区及颞叶皮质HSP70的表达。结果 与P、C组比较,E组双侧海马和颞叶皮质HSP70的表达均有不同程度下降(P<0.05),且双侧之间改变无明显差异(P>0.05)。结论持续左侧SGB,可降低全脑缺血再灌注损伤家兔神经细胞HSP70的过度表达,单侧SGB的效应对于双侧脑组织损伤后的影响差异无显著性。
Objective To investigate the effects of unilateral stellate ganglion block (SGB) on the expression of heat shock protein 70(HSP70) in bilateral hippocampus and temporal lobe induced by global cerebral ischemia-reperfusion in rabbits. Methods Twenty-eight healthy Japanese long-ear rabbits of either sex weighing 2.5-3.0 kg were anesthetized with intravenous 1.5% pentobarbital, intubated and mechanically ventilated. Global cerebral ischemia was produced by clamping bilateral external and internal carotid arteries and vertebral arteries for 10 min. Global cerebral ischemia was confirmed by blanching of lips and conjunctival, respiratory and EGG changes. The arteries were then declamped for reperfusion. The animals were randomly divided into 4 equal groups ( n = 7) : SGB group, NS control group, blank control group and sham operation group. In SGB group left stellate ganglion was blocked by 0.25% bupivacaine 0.5 ml injected through an epidural catheter with its tip placed close to left stellate ganglion when reperfusion was started followed by continuous infusion of 0.25 % bupivacaine at a rate of 0.5 ml · h -1 . SGB was confirmed by ptosis and miosis. In NS control group normal saline was used instead of 0.25 % bupivacaine. In blank control group no medication was give. In sham operation group the six arteries were exposed but not occluded. The HSP70 expression in the neurons in hippocampus CA1 section and cortex of temporal lobe was detected by immunohistochemistry using an antibody specific for HSP70 30h after reperfusion was started. Results HSP 70 expression in bilateral hippocampus CA1 section and cortex of temporal lobe was significantly lower in SGB group compared with the two control groups ( P < 0.05), but there was no significant difference in HSP 70 expression between the two cerebral hemispheres ( P > 0.05) . Conclusion Left SGB can depress the over-expression of HSP 70 in bilateral hippocampus and temporal lobe induced by global cerebral ischemia-reperfusion.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2004年第2期133-137,共5页
Chinese Journal of Anesthesiology
基金
湖北省教育厅优秀中青年人才基金项目(编号2000843001)
