摘要
目的:本实验建立小鼠脑缺血模型,观察黄芪对小鼠脑缺血热休克蛋白70的表达影响。方法:将48只健康昆明小鼠随机分为三组:假手术组、缺血组、黄芪组。黄芪组腹腔内注射黄芪注射液2.5g/kg,缺血组和假手术组腹腔内注射等量生理盐水,连续7天后用微动脉夹夹闭小鼠双侧颈总动脉20min,手术后即刻断头取脑、冷冻,进行热休克蛋白70的定量分析。结果:与假手术组相比,缺血组热休克蛋白70的表达升高58%,黄芪组热休克蛋白70的表达升高32%;与缺血组相比,黄芪组热休克蛋白70的表达降低16.5%。结论:脑缺血敏感地诱导了热休克蛋白70的表达,而黄芪的应用明显地抑制了其表达。
Objective：This experiment establishes the mouse cerebral ischemia model to observe astragalus how to influence the expression of HSP 70. Methods： 48 health Kunming mice were divided into three groups at random： Sham group, ischemia group, astragalus group. Mice of astragalus group were injected astragalus inoculation fluid 2. 5g/kg into abdominal cavity, others were injected the isometric physiological saline ;Td later, all mice were blocked bilateral common carotid artery with artery clip for 20. then were taken the brain tissue, frozen and carried on the HSP70 quantitative analysis. Results： Compared with the sham group, the ischemia group HSP70 expressions increased 58% , the astragalus group HSP70 expressions increased 32% ; Compareed with the ischemia group, the astragalus group HSP70 expressions reduced 16.5%. Conclusion： The cerebral ischemia has induced the HSP70 expressions sensitively, but the astragalus application has suppressed its expression obviously.
出处
《中国医药导刊》
2008年第4期607-607,613,共2页
Chinese Journal of Medicinal Guide
