摘要
目的 :探讨冷冻外科以及冷冻配合免疫疗法控制小鼠Lewis肺癌的效果。方法 :将小鼠Lewis肺癌细胞株接种至C57BL/ 6小鼠皮下。当肿瘤长至直径 0 .5± 0 .1cm时 ,随机分为四组 ,分别是A组 (冷冻组 )、B组 (免疫组 )、C组 (冷冻配合免疫组 )、D组 (对照组 )。观察治疗后小鼠肿瘤体积变化和存活期。另外 ,对治疗后 10d小鼠进行NK细胞活性检测 ,判断冷冻外科对小鼠免疫机能的影响。结果 :冷冻治疗后 ,Lewis肺癌体积在 1周内出现明显缩小。术后 2 1d ,A、B、C及D组的平均肿瘤体积分别是 1.0 0、1.2 6、0 .35和 2 .85cm3 。A、B、C及D组的存活期分别是 5 2 .1d± 3.2 2d、33.5d± 2 .0 7d、6 9.9d± 7.5 8d及 2 9.1d± 1.79d。C组及A组的存活期均明显优于D组 (两组比较均为P <0 .0 5 )。另外 ,术后 10dC组小鼠NK活性为 4 1.4 % ,均明显高于D组 (P<0 .0 1)。而A组小鼠NK活性和D组无明显差别。结论 :冷冻外科可以有效地控制Lewis肺癌的生长 ,延长荷瘤小鼠存活期。冷冻配合IL 2协同治疗更加有效提高围手术期NK细胞活性。
Objective: To assess the effects of cryosurgery and cryosurgery combined with immunological treatment in Lewis lung cancer model, measure the cytotoxicity of NK after cryosurgery and discuss the influence of tumor-immunology. Methods: C_ 57 BL/6 mice were inoculated subcutaneous with Lewis lung cancer cells. When the tumor grew to 0.5 cm±0.1 cm in diameter, the tumor-bearing mice were divided into 4 groups.A. Cryosurgery group:the mice were tread twice by -60℃ freezer;B. Immunotherapy group: the mice were daily injected with IL-2(10 000U), for 7 days;C Cryo-iummunotherapy group: the mice were tread by -60℃ freezer and daily injected with IL-2(10 000U),for 7 days. C.Control group: untreated. The therapeutic effect was assessed by tumor size and survival time. Non-Radioactive Cytotoxicity Assay were chosen to examine the cytotoxicity of NK. Results: On day 21,the average tumor size of the 4 groups of mice was 1.00?1.26?0.35 and 2.85 cm 3 respectively. The average surviving time of the 4 groups of mice were 52.1±3.22?33.5±2.07?69.9±7.58 and 29.1±1.79 days respectively. Group A and group C showed statistically significant difference with the control group (both P <0.05). In vitro,cytotoxicity of NK cells level of group C was 41.4% which was significant higher than control group ( P <0.01). Conclusion: The mice tumor model demonstrates that cryosurgery is effective in control tumor growth and prolongs surviving time of Lewis lung cancer. Cryosurgery combined with IL-2 can stimulate cytotoxicity level of NK during perioperate period and possibly enhance a systemic, specific anti-tumor immune response.
出处
《中日友好医院学报》
2004年第2期92-95,共4页
Journal of China-Japan Friendship Hospital
