摘要
目的 探讨微脉管密度 (TMVD )、微血管密度 (BMVD)、微淋巴管密度 (LMVD )与乳腺癌临床病理特征的关系。方法 选取 80例临床病理资料完整的乳腺癌组织 ,应用免疫组化双染试剂盒 (Histostain DS试剂盒 )、FVⅢ RA单克隆抗体和Ⅳ型胶原单克隆抗体 ,在同一张组织切片上分别对内皮细胞和基膜进行标记 ,在光镜下分别观察微脉管密度、微血管密度和微淋巴管密度。结果 微脉管密度在腋窝淋巴结阴性组和淋巴结转移 1～ 3个组、4～ 9个组、10个以上组分别为 3 5 .64± 9.2 8,45 .47± 7.60 ,5 7.0 0± 9.17,67.0 0± 9.5 6;微血管密度在以上各组分别为 2 3 .3 6± 7.0 6,3 0 .5 3± 6.13 ,3 8.40± 6.67,43 .75± 6.85 ;微淋巴管密度在以上各组分别为 12 .2 7± 2 .49,14 .93± 2 .46,18.60± 2 .76,2 3 .2 5± 3 .40。微脉管密度、微血管密度和微淋巴管密度均与腋窝淋巴结状态呈显著正相关 (P <0 .0 5 ) ,随腋窝淋巴结转移数目的增加而增大 ;而三者与肿瘤大小、病理类型、组织分化程度、绝经状态以及雌激素受体状况等因素均无相关性 (P >0 .0 5 )。结论 微脉管密度、微血管密度、微淋巴管密度与乳腺癌腋窝淋巴结状态密切相关 。
Objective To investigate the relationship between lymphatic microvessel density,blood microvessel density and breast cancer′s clinical and pathological features.Methods The data of 80 cases of breast cancer were collected.Two monocolonal antibodies,anti-FVⅢ-RA monocolonal antibody and anti-collagen Ⅳ monocolonal antibody were used to mark endotheliocyte and basement membrane.Then the peritumor lymphatic microvessel density(LMVD),blood microvessel density(BMVD)and total microvessel density(TMVD)were observed under microscope.Results TMVD was 35.64±9.28 in patients without lymph node metastasis,45.47±7.60,57.00±9.17 and 67.00±9.56 in patients with 1～3,4～9,and ≥10 axillary lymph nodes metastasis,respectively.BMVD was 23.36±7.06,30.53±6.13,38.40±6.67 and 43.75±6.85 in the above four groups,respectively.LMVD was 12.27±2.49,14.93±2.46,18.60±2.76,23.25±3.40 in the above four groups,respectively.LMVD,BMVD and TMVD had significantly positive correlation with the status of axillary lymph node(P<0.05),but had no correlation with the size,pathological type of tumor,differentiation degree,and status of estrogen receptor et al(P>0.05).Conclusion TMVD,BMVD and LMVD are important indicators to evaluate the status of axillary lymph node of breast cancer.
出处
《实用癌症杂志》
2004年第2期144-147,共4页
The Practical Journal of Cancer
关键词
乳腺癌
癌周组织
微血管密度
病理特征
相关性
肿瘤转移
Breast cancer
Peritumor
Total microvessel density
Clinical and pathological features
Status of axillary lymph node
