摘要
目的 评价南昌地区幽门螺杆菌 (Helicobactcrpylori,Hp)表型在胃十二指肠疾病中的分布情况 ,并探讨其临床意义。方法 2 0 6例慢性胃炎 (CG)、消化性溃疡 (PU)和胃癌 (GC)的南昌患者经胃镜和组织学检查确诊 ,用免疫印迹法检测Hp细胞毒素相关蛋白 (CaSA)、空泡毒素 (VacA)和尿素酶 (Urease)及其亚型。结果 Hp感染人群中Hp表型CaZA、VacA和Urease的表达率分别为 5 7.1%、78.6 %和 10 0 %。Hp表型CagA、VacA及亚型 12 8KDCagA、116KDCagA、95 1KDVacA、91KDVacA、6 6KDUreB、30KDUreA ,无一在CG组、PU组和GC组中的表达率差异有显著性 (P >0 .0 5 )。表型CagA +/VacA +(CagA +或 /和VacA +)在CG组、PU组和GC组的表达率分别为 74 .4 %、85 .7%和 94 .4 % ,在CG组、PU组和GC组中 ,P =0 .0 2 4 ;在CG组和PU组中 ,P =0 .14 5 ;CG组和GC组中 ,P =0 .0 11:在PU组和GC组中 ,p =0 .2 75 ;在非CC(即CG和PU)组和CC组中 ,P =0 .0 2 4。116KDCagA在≤ 4 5岁患者的表达率 (6 0 .5 % )高于 >4 5岁患者的表达率 (42 .7% ) (P =0 .0 37)。结论 在Ca gA+ 南昌地区的Hp感染人群中 ,Hp的CaSA表达率明显低于VacA。Hp单一表型和亚型未显示其独立的致病作用。表型CagA+ /VacA +与GC有关 。
Objective: To evaluate the distribution of H. pylori phenotypes among gastroduodenal diseases in Nanchang area and explore their clinical significance.Methods:206 patients from Nanchang area were diagnosed as chronic gastritis (CG), peptic ulcer (PU) and gastric cancer (GC) by endoscopy and histology. Western blotting was used to detect phenotype of H. pylori cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), Urease and their sub-phenotypes. Results:The expression rate of phenotype of H. pylori CagA, VacA and Urease in the infecting strain population was 57.1%,78.6% and 100%, respectively. No significance was observed among the expression rate of phenotype CagA, VacA and sub-phenotype 128KD CagA, 116KDCagA, 95KDVacA, 91KDVacA, 66KDUreB and 30KDUreA in group CG, PU and GC ( P >0.05). The rate of phenotype CagA +/VacA + (i.e., CagA + and / or VacA +) in group CG, PU and GC was 74.4%, 85.7% and 94.4%, respectively; among CG, PU and tiC, P =0.024; between group CG and PU, P =0.145;between group CG and GC, P =0.011; between group PU and GC, P =0.275; between group non- GC (i.e., CG and PU) and GC, P =0.024. The expression rate of 116KDCagA in patients ≤45 yr of age(60.5%) was higher than that of >45 yr of age(42.7%)( P =0.037). Conclusions:The expression rate of H. pylori CagA in the infecting strain population from Nanchang area is obviously lower than that of VacA. Single phenotype or sub-phenotype does not seem to play an independent pathogenic role. Phenotype CagA +/VacA + is related to GC, indicating H. pylori probably cause formation of GC by comprehensive effect of many phenotypes and others not mentioned in present paper. 116KDCagA is related to age, but its signification is not clear.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第9期25-29,共5页
China Journal of Modern Medicine
关键词
幽门螺杆菌
表型
免疫印迹法
慢性胃炎
消化性溃疡
胃癌
helicobacter pylori
phenotype
western blotting
chronic gastritis
peptic ulcer
gastric cancer
