摘要
涂敷直链淀粉 三(3,5 二甲基苯基氨基甲酸酯)(ADMPC)于自制的球形氨丙基硅胶上,制备了一种手性固定相。采用高效液相色谱法(HPLC),在正相条件下用该固定相直接拆分了广谱驱虫药物阿苯达唑的代谢产物阿苯达唑亚砜(albendazolesulfoxide,ABZSO)的外消旋对映体。系统地选用了多种二元及三元流动相体系对样品进行拆分,结果表明,流动相中不同种类的醇改性剂及其含量的不同对样品的保留时间和立体选择性有不同程度的影响,甲醇、乙醇等作改性剂用于拆分样品的效果较好;采用三元流动相体系正己烷 异丙醇 甲醇或正己烷 乙醇 甲醇可以实现对ABZSO快速有效的手性拆分。
A chiral stationary phase (CSP) was prepared by coating amylose-tris ( 3,5-dimethylphenyl carbamate ) (ADMPC) onto aminopropylated spherical silica gel. Albendazole sulfoxide (ABZSO) was directly resolved on the ADMPC-CSP by normal-phase high performance (liquid) chromatography (HPLC) using a series of binary and ternary mobile phases. The results (indicated) that the retention and stereoselectivity of ABZSO on ADMPC-CSP were significantly (influenced) by the kinds and the contents of the alcohols in mobile phases. The use of methanol and/or ethanol as alcoholic modifier achieved better separation of ABZSO. Effective and efficient chiral resolution was obtained by using ternary mobile phases: hexane/2-propanol/methanol or hexane/ethanol/methanol.
出处
《色谱》
CAS
CSCD
北大核心
2004年第3期244-247,共4页
Chinese Journal of Chromatography
基金
国家科技部重大基础研究快速反应项目和中国科学院"百人计划"项目.
