摘要
将禽流感病毒A/Goose/Guangdong/3/96(H5N1)毒株的HA和NA2个基因同源重组到禽痘病毒基因组中,获得了能同时高效表达这2种蛋白的重组禽痘病毒(rFPV-HA-NA)。将rFPV-HA-NA经翅膀刺种途径接种8周龄SPF鸡,免疫后4周分别用10LD50的高致病力禽流感病毒(HPAIV)A/Goose/Guangdong/1/96(H5N1)和A/FPV/Rostock/34(H7N1)毒株进行攻击。结果重组禽痘病毒免疫鸡群诱导产生了高水平的抗体,能够完全抵抗H5N1和H7N1亚型病毒的致死性攻击;并可有效阻止病毒在泄殖腔的排出。而禽痘病毒免疫组和非免疫对照组在攻毒后全部发病并死亡。
A recombinant fowlpox virus co-expressing haemagglutinin (HA) and neuraminidase (NA) named as rFPV-HA-NA was produced by HA and NA gene of A/Goose/Guangdong/3/96 (H5N1) isolate of avian influenza virus recombinedinto the genome of fowlpox virus. In this study, to evaluate its ability to protect chickens against challenge with a lethal doseof highly pathogenic isolates of avian influenza virus, eight-week-old specific-pathogenic-free (SPF) chickens were vaccinatedwith recombinant virus or the wildtype fowlpox virus by wing-web puncture. Following challenge 4 weeks later with 10LD50highly pathogenic avian influenza virus H5N1 and H7N1 isolate, all chickens vaccinated with recombinant virus wereprotected, while the chickens vaccinated with the wildtype fowlpox virus or unvaccinated controls experienced 100%mortality respectively following challenge. And this complete protection was accompanied by the high levels of specificantibody response to the respective components of the recombinant virus.
出处
《中国农业科学》
CAS
CSCD
北大核心
2004年第4期605-608,共4页
Scientia Agricultura Sinica
基金
国家"863"高技术研究发展计划资助项目(2001AA213041)
