摘要
将分别来自禽流感病毒A/Goose/Guangdong/3/96(H5N1)毒株的HA基因和A/Goose/Guangdong/1/96(H5N1)NP基因重组到禽痘病毒基因组中,获得了能同时高效表达这两种蛋白的重组禽痘病毒(rFPV HA NP)。将rFPV HA NP经翅膀刺种途径接种8周龄SPF鸡,并设亲本禽痘病毒免疫和非免疫对照组。免疫后4周分别用10LD50的高致病力禽流感病毒(HPAIV)A/Goose/Guangdong/1/96(H5N1)和A/FPV/Rostock/34(H7N1)毒株进行攻击。结果重组禽痘病毒rFPV HA NP免疫鸡群后能够诱导产生高水平的特异性抗体,可完全抵抗H5N1毒株的致死性攻击,并可有效阻止H7N1病毒攻击后病毒在泄殖腔的排出。而禽痘病毒免疫组和非免疫对照组在攻毒后全部发病并死亡。结果表明NP在与HA共同表达时,在诱导交叉保护性方面有一定的免疫增强功能。
A recombinant fowlpox virus co- expressing Haemagglutinin(HA) and Nucleoprotein(NP) named as rFPV- HA- NP was produced by HA gene of A/Goose/Guangdong/3/96(H5N1) and NP gene of A/Goose/Guangdong/1/96(H5N1) isolate of avian influenza virus recombined into the genome of fowlpox virus.In this study,to evaluate its ability to protect chickens against challenge with a lethal dose of highly pathogenic isolates of avian influenza virus,eight- week- old specific- pathogen- free(SPF)chickens were vaccinated with recombinant virus or the parent fowlpox vaccine virus by wing- web puncture.Following challenge 4 weeks later with 10LD(50) highly pathogenic avian influenza virus H5N1 and H7N1 isolate,all chickens vaccinated with recombinant virus were protected against the challenge with H5N1,and were effectively inhibited to shed viruses in cloaca when challenge with H7N1,while the chickens vaccinated with the unaltered parent fowlpox vaccine virus or unvaccinated controls experienced 100% mortality respectively following challenge.And these protections were accompanied by the high levels of specific antibody response to the respective components of the recombinant virus.The above results indicated that NP protein would enhance the immuogenicity of the vaccine at cross- reactivity level.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2004年第2期178-181,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
基因表达
禽流感
HA
NP
双基因重组
禽痘病毒
免疫保护性
Avian Influenza Virus
Haemagglutinin
Nucleoprotein
Recombinant Fowlpox Virus
Protective Immunity
