摘要
前期研究观察到一种现象 ,在正常妊娠的胎盘中细胞粘附分子CD14 6选择性地表达在侵入性滋养层细胞中 ,而在滋养层细胞侵入不足的先兆子痫病人的胎盘中CD14 6表达降低或缺失 .本文进一步研究了CD14 6分子影响滋养层细胞侵入行为的作用机理 .免疫荧光实验显示CD14 6分子选择性地表达在具有侵袭能力的中间滋养层细胞 ,而在非侵入性的细胞滋养层细胞和合体滋养层细胞中不表达 .细胞功能实验表明 ,影响滋养层细胞侵入性的两个关键要素 ,即细胞迁移和基质金属蛋白酶的分泌 ,都受到CD14 6特异抗体的显著抑制 .这些研究结果提示 ,粘附分子CD14 6是影响细胞侵入行为的关键分子 .这为深入研究胚胎植入和肿瘤浸润的分子调控机理提供了一个关键的分子模型 .
Previous study has shown that melanoma cell adhesion molecule (MCAM/CD146) is highly expressed by invasive trophoblasts in normal pregnancy, but it is absent in pregnancy disorder pre-eclampsia in which the invasion ability of trophoblast is seriously impaired. The mechanism that CD146 affects the trophoblast invasion was investigated. Immunofluorescence staining revealed that CD146 was selectively expressed on invasive intermediate trophoblasts, but was not expressed on non-invasive cytotrophoblasts and syncytiotrophoblasts. Functional studies showed that the anti-CD146 antibody significantly inhibited the migration of trophoblasts and the activity of matrix metalloproteinases secreted by trophoblastic cells, which are two critical factors for trophoblast invasion. The results demonstrate that adhesion molecule CD146 plays a critical role in trophoblast invasion, which will provide a valuable molecular model for the study of trophoblast and tumor cell invasion.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第4期309-312,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目 ( 3 0 0 70 3 87)
国家高技术"863计划"资助项目 ( 2 0 0 1AA2 15 3 2 1)~~
