摘要
目的 利用亚硝基乙基脲 (ENU)经胎盘诱发大鼠脑肿瘤的模型 ,对肿瘤发生前、后各阶段p16蛋白表达进行连续动态观察。方法 对该模型出生后 6 0d、90d、12 0d、15 0d的子代鼠脑进行p16蛋白免疫组化染色观察。结果 (1) 6 0d组的p16蛋白均表达阳性 ,无缺失发生 ;90d组偶有阴性 ;12 0d组阳性率下降 ;15 0d组阳性率明显下降。瘤周组织的阳性表达率高于瘤内组织。 (2 )p16蛋白主要定位于细胞核 ,极少部分定位于细胞浆。结论 (1)随着本模型肿瘤发生率的增加 ,大鼠脑内p16蛋白的表达下降 ,说明p16基因突变过程正是肿瘤的发生发展时期 ,p16基因活动下降与本模型胶质瘤的发生发展有关。(2 )p16蛋白定位主要位于细胞核内 。
Objective To study in series the p16 protein expression on the rat brain tumor induced transplacentally by ENU Methods The p16 protein expression was determined with immuno histochemistry stain on the offspring′s brain at their 60,90,120,150 days after birth Results (1) p16 proteins were expressed in all of the brain samples in the 60 day group; occasionally negative in the 90 day group; partly expressed in the 120 day group; significantly less expressed in the 150 day group (2) The rate of expression in the tissue around tumor was higher than that in the tumor (3) The p16 protein was mainly orientated in the nuclear of cell and sporadically orientated in the cytoplasm Conclusion (1)It shows the p16 protein expression decreases with the increase of tumor incidence in the rat brain, which accompanies the start and development of the induced tumor So we speculate that the dysfunction of p16 gene is one of the factors related to tumor incidence in this animal model (2) The p16 protein is mainly orientated in the nuclear of cell and sporadically orientated in the cytoplasm
出处
《中华外科杂志》
CAS
CSCD
北大核心
2004年第6期358-361,共4页
Chinese Journal of Surgery
基金
国家自然科学基金资助项目(39770751)
