摘要
目的 探讨血管内皮生长因子 (VEGF)血小板反应素 1(TSP 1)和微血管计数 (MVC)在眼肿瘤病人组织中的表达及其关系。方法 采用逆转录聚合酶链反应法 (RT PCR)和免疫组化S P法检测VEGF、TSP 1和MVC在 90例眼肿瘤病变组织中的表达 ,并用计算机图像分析系统对血管新生的强度进行定量分析。结果 肿瘤组织VEGF和MVC的表达同步增高 ;TSP 1mRNA的表达和MVC、VEGF mRNA有显著的负相关 (r =- 0 4 5 2 ,r=- 0 4 4 7,P均 <0 0 5 ) ;VEGFmRNA表达与MVC之间呈显著的正相关 (r =0 4 5 2 ,P <0 0 5 )。结论 眼肿瘤病人组织中VEGF及MVD的表达 ,可能与其血管化程度有关 ;TSP 1在肿瘤血管抑制方面发挥重要的作用。
Objective To determine the correlation of vasvular endothelial growth factor(VEGF),thrombospondin 1(TSP 1)mRNA and microvessed counts(MVC) in eye tumor.Methods The expression of VEGF,TSP 1 mRNA and MVC in 90 eye tumor were studied with S P immunohistochemical technique and reverse transcription polymerase chain reaction(RT PCR),the extent of neovascularization was assessed by computer assisted video analysis system.Results The expression of tumor the VEGF mRNA synchronously increased with the MVC;The expression of TSP 1 mRNA was negatively associated with MVC,VEGF mRNA(r=-0.452,r=-0.447,the P is all <0.05).The expression of VEGF mRNA was positively linked to MVC(r=0.452,P<0.05).Conclusion The expression of VEGF and MVC in eye tumor is associated with its neovelscualization extent.
出处
《中国基层医药》
CAS
2004年第3期309-310,共2页
Chinese Journal of Primary Medicine and Pharmacy
