摘要
目的 检测单核细胞趋化蛋白 1(MCP 1)在子宫内膜异位症 (内异症 )患者子宫内膜组织中的表达情况 ,并探讨其与内异症发病的关系。方法 内异症患者 30例作为研究组 ,非内异症患者 2 0例作为对照组。用半定量逆转录聚合酶链反应 (RT PCR)方法检测两组子宫内膜组织中MCP 1信使核糖核酸 (mRNA)的阳性表达率及相对水平。结果 研究组 2 6例 (87% )内膜组织中可检测到MCP 1mRNA的表达 ,其中 19例为强阳性表达 ,但其表达强度与内异症的严重程度无明显相关性。对照组 15例 (75 % )内膜组织中可检测到MCP 1mRNA表达 ,仅 3例为强阳性表达。研究组内膜组织中MCP 1mRNA的表达强度高于对照组 (两组相对表达强度分别为 0 746± 0 345及 0 46 0± 0 341,P <0 0 5 )。结论 内异症患者的子宫内膜组织中MCP 1mRNA的表达增强 ,MCP
Objective To examine the expression of monocyte chemotactic protein 1 (MCP 1) messenger ribonucleic acid (mRNA) in the endometrium of patients with and without endometriosis Methods A reverse transcription polymerase chain reaction ( RT PCR) method was utilized to examine and quantitate MCP 1 mRNA expressions and levels in the eutopic endometrium Thirty patients with endometriosis were included as study group, and 20 patients without endometriosis selected as control group Results 26 (87%) of 30 samples of endometrium from patients with endometriosis expressed MCP 1 mRNA, 19 of which were strong expression, while 15 (75%) of 20 samples of endometrium from patients without endometriosis express MCP 1 mRNA ( P >0 05) When endometriosis were grouped by Revised American Fertility Society (RAFS) stages, there was no obvious correlation between stage and MCP 1 mRNA expression level ( P >0 05) Semi quantitative PCR showed the expression level of MCP 1 in endometrium of patients with endometriosis were higher than those of patients without endometriosis (0 746±0 345 Vs 0 460±0 341 respectively, P <0 05) Conclusions The MCP 1 mRNA is over expressed in the eutopic endometrium from patients with endometriosis, which may play some roles on the pathogenesis of endometriosis.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2001年第2期89-91,共3页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金资助项目(39830350)
