摘要
目的 :研究腓肠神经移植替代损伤的双侧海绵体神经恢复大鼠的勃起功能。 方法 :4 8只雄性SD大鼠 (3～ 4月龄和 30 0～ 4 0 0g)随机分为神经移植组、神经损伤组及假手术组 ,每组 16只。 2、4个月后 ,海绵体神经电刺激检测大鼠阴茎勃起功能 ,阴茎内注射神经逆行示踪剂荧光金 5d后检测盆神经节内被标记的神经元细胞。 结果 :2个月后神经移植组与神经损伤组大鼠对海绵体神经电刺激均无勃起反应 ,两组盆神经节内荧光金标记的神经元细胞数目差异有显著性 (P <0 .0 5 ) ;而 4个月后神经移植组大鼠勃起功能较神经损伤组差异有显著性 (P <0 .0 5 ) ,盆神经节内荧光金标记的神经元细胞数目也显著高于神经损伤组 (P <0 .0 5 ) ,与假手术组差异无显著性 (P >0 .0 5 )。 结论 :腓肠神经移植替代损伤的双侧海绵体神经可恢复大鼠的勃起功能。
Objective: To investigate the restoration of erectile function by reconstructing cavernous nerves with sural nerve grafts. Methods: Forty-eight male Sprague-Dawley rats(3～4 m old and 300～400 g) were randomly divided into three groups: the sham-operated group (n=16) underwent pelvic exploration without transection of the cavernous nerve; the nerve ablation group (n=16) had a 5 mm segment of the cavernous nerve excised bilaterally; the graft group(n=16) had a 5 mm segment of the cavernous nerve excised bilaterally, followed by immediate microsurgical reconstruction with an interposition graft of the sural nerve. The cavernous nerves of each group were electrostimulated to determine their potency after 2 and 4 months. And fluorescent retrograde-transported material Fluoro-Gold(FG) was injected into the penis. FG-labeled neuron cells in whole mounts of major pelvic ganglions were observed five days after injection. Results: Electrical stimulation produced no erection in either the nerve ablation or the graft group, but 100% erection in the sham-operated group after 2 months. The numbers of FG-labeled neurons significantly differed between the nerve ablation group and the graft group. After 4 months erection examination showed statistical significance in the difference between the graft group and the nerve ablation group(P< 0.05). The FG-labeled neurons in the graft group significantly differed from those in the ablation(P< 0.05), and almost reached the level of the sham-operated(P> 0.05). Conclusion: Cavernous nerve grafting can successfully restore erectile dysfunction in rats after surgical injury.
出处
《中华男科学杂志》
CAS
CSCD
2004年第5期330-333,共4页
National Journal of Andrology
基金
国家自然科学基金资助 ( 3 0 170 93 9)
关键词
海绵体神经
移植
勃起功能
逆行示踪
大鼠
cavernous nerve
graft
erectile function
retrograde labeling
rat
