摘要
目的 :分析神经肽Y(NPY)在大鼠睾丸中的表达 ,探讨NPY在雄激素生成和精子发生中的作用。 方法 :用RT PCR半定量法分析SD雄性大鼠睾丸中的NPYmRNA的表达 ,以β actin为内参照。用免疫组化法观察NPY在组织和细胞内的分布。 结果 :NPY基因在大鼠睾丸中有相当水平的表达 ,在PCR产物电泳图谱中见到明显的基因扩增产物条带 ;免疫组化显示 ,NPY在睾丸间质区、睾丸小血管周围及其精曲小管周围阳性表达 ,而在精曲小管内未见表达。 结论 :睾丸内表达的NPY可能直接参与了睾丸功能的调节。
Objective: To assay the expression of neuropeptide Y (NPY) in rat testes and to investigate the significance of NPY in the regulation of androgen production and spermatogenesis. Methods: NPY mRNA levels in SD rat testes were measured by RT-PCR semi-quantity with β-actin as internal control. NPY distribution was observed immunohistochemically. Results: NPY gene expressed in the testes, showing the strong positive band in the PCR production electrophoresis gel. In the immumostaining slides, NPY was found positively expressed in the Leyding cell area and around the testicular vessels and tubules, but not in the seminiferous tubules. Conclusion: There was positive expression of NPY in the rat testes, which showed that NPY played a direct role in the regulation of testicular function.
出处
《中华男科学杂志》
CAS
CSCD
2004年第5期334-336,339,共4页
National Journal of Andrology
基金
江苏省 13 5重点医学人才项目 (RC2 0 0 2 0 76)
关键词
神经肽Y
睾丸
表达
大鼠
neuropeptide Y
testis
expression
rat
