摘要
目的 探讨用生物传感技术测定多粘菌素B(PMB)中和内毒素活性物质能力的可行性。 方法 将PMB与一定浓度的内毒素混合后 ,分别用生物传感技术和鲎试验法测定PMB中和内毒素能力的比值 ,同时与用生物学法检测的结果进行比较。 结果 用生物传感技术测定PMB中和内毒素能力的比值为 0 .35 μg∶1ng,动态浊度法为 0 .5mg∶1ng、基质显色法为 1.0mg∶1ng,生物学测定结果与生物传感技术测定结果相同。 结论 应用生物传感技术对PMB中和内毒素活性物质的能力进行测定 ,方便、快速、准确 ,具有实用价值。
Objective To explore the application of biosensor technology in the determination of endotoxin-neutralizing materials. Methods After mixing polymyxin B (PMB) with endotoxin in certain concentration, the neutralizing ratio of PMB to endotoxin was assessed by biosensor technique and limulus amebocyte lysate test respectively. The results from the two methods were compared. Results The neutralizing ratio of PMB to endotoxin as assessed by biosensor technology was 0.35 μg to 1 ng , while that by dynamic turbidimetric and chromogenic limulus amebocyte lysate(LAL) technique was 0.5mg to 1ng and 1mg to 1 ng, respectively. The results obtained by biotechnology were similar to that by biosensor technique. Conclusion Biosensor technology was an accurate, convenient and rapid method for the determination of potency of endotoxin-neutralizing materials.
出处
《中华烧伤杂志》
CAS
CSCD
2004年第1期23-25,共3页
Chinese Journal of Burns
