摘要
目的 研究在中国南方汉人群中 ,血管紧张素原基因 (angiotensinogen ,AGT)启动子区域 2 17位和 2 0位上的二种单核苷酸多态与高血压病 (EH)并发冠心病的关系。方法 运用多重SNaPshot反应 ,对 2 0 5例EH并发冠心病患者、185例EH患者和 185名健康对照者进行G 2 17A和A 2 0C多态基因分型。结果 G 2 17A多态的基因型分布在EH并发冠心病组 (AA =8、AG =71、GG =12 6 )和对照组 (AA =8、AG =37、GG =14 0 )之间有显著性差异 (P =0 0 0 5 ) ;A、G等位基因频率与对照组相比亦有显著性差异 (A 2 1 2 2 %、G 78 78%比A 14 32 %、G 85 6 8% ,P =0 0 12 ) ;A 2 0C多态的基因型分布 (CC、AC、AA)及C、A等位基因频率在二组间的差异无显著性 (分别为CC =5、AC =4 9、AA =15 1比CC =2、AC =6 1、AA =12 2 ,P =0 0 97;C 14 39%、A 85 6 1%比C 17 5 7%、A 82 4 3% ,P=0 2 2 6 )。在男性EH并发冠心病组中 ,G 2 17A和A 2 0C多态的基因型分布及其等位基因频率与对照组相比均有显著性差异 (G 2 17A :AA =7、AG =5 3、GG =86比AA =6、AG =2 8、GG =97,P =0 0 2 2 ;A 2 2 95 %、G 77 0 5 %比A 15 2 7%、G 84 73% ,P =0 0 2 2。A 2 0C :CC =3、CA =2 7、AA =116比CC =2、CA =4 3、AA =86 ,P =0 0 2 3;C 11 30 %
Objective To evaluate the relationship between two single nucleotide polymorphisms(SNPs) at position -217 and -20 in the promoter region of angiotensinogen(AGT) gene and essential hypertension(EH) complicated by coronary heart disease(CHD) in south han Chinese population. Methods ABI PRISM SNaPshot Multiplex Kit was used to genotyping G-217A and A-20C polymorphisms in 205 patients with EH complicated by CHD,185 patients with EH and 185 healthy controls. Results The genotype distribution of G-217A polymorphism was significantly different between group of EH complicated by CHD (AA=8,AG=71,GG=126)and group of controls (AA=8,AG=37,GG=140, P=0.005). Frequencies of A and G alleles of G-217A were also obviously different between two groups (A 21.22%,C 78.78% vs. A 14.32%,C 85.68%,P=0.012).However,the genotype distribution(CC,AC and AA) and frequencies of C and A alleles of A-20C polymorphism had no difference between two groups(CC=5,AC=49,AA=151 vs. CC=2,AC=61,AA=122,P=0.097;C 14.39%,A 85.61% vs. C 17.57%, A 82.43%,P=0.226).The genotype distribution and allele frequencies of G-217A and A-20C polymorphisms were obviously different between two male groups(G-217A:AA=7,AG=53,GG=86 vs. AA=6,AG=28,GG=97, P=0.022; A 22.95%, G 77.05% vs. A 15.27%, G 84.73%, P=0.022. A-20C:CC=3, AC=27, AA=116 vs. CC=2, AC=43,CC=86,P=0.023;C 11.30%, A 88.70% vs. C 17.94%, A 82.06%, P=0.026).The genotype distribution of G-217A was also obviously different between group of double-vessel(DVD) and triple-vessel disease(TVD) and group of controls (AA=2,AG=40,GG=67 vs. AA=8, AG=37, GG=140,P=0.005). Conclusion G-217A polymorphism of AGT gene might be a genetic risk factor for CHD in patients with EH. A-20C polymorphism might be associated with the occurrence of coronary heart disease in male patients with EH but not in female. G-217A polymorphism might be also associated with the extent of coronary atherosclerosis.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2004年第4期317-321,共5页
Chinese Journal of Cardiology
