摘要
原子力显微镜 (AFM)对完整的细胞成像并同时进行微细结构观察尚有一定困难。本实验改进了标本制备的过程 ,用原子力显微镜对戊二醛固定的海马神经元进行扫描 ,建立了方便、实用的完整海马神经元自完整胞体至超微结构的原子力显微镜成像技术 ,并用改进的方法获得了完整海马神经元及其超微结构的清晰的三维成像 ,并发现了一些其它显微技术所不能发现的微细结构。这些结构包括 :①海马神经元胞体的亚细胞部分及这些亚细胞部分所具有的不同功能 ;②神经突触的完整形态 ;③损伤细胞膜表面出现的孔洞 ;
The atomic force microscope (AFM), with its unique high resolution capability, plays an ever-increasingly important role in the field of biological research. Yet, with the difficulty in scanning the whole cells, its application is restricted. In this study, we have found an appropriate way to observe the glutaraldehyde-fixed hippocampal neurons from cell bodies to fine structures with AFM. By refining the technique of specimen preparation,three-dimensional images of the whole hippocampal neuron, neuronal membrane surface, neurites, synapses and some other fine structures were observed with AFM. The result of this study suggests that the newly established method of specimens preparation may provide high quality three-dimensional images of the intact hippocampal neurons as well as their surface fine structures. Some structures that are difficult to be found by other microscopy also can be observed with AFM.
出处
《电子显微学报》
CAS
CSCD
北大核心
2003年第2期125-130,共6页
Journal of Chinese Electron Microscopy Society
基金
军队十五规划科研基金重点课题 (No 0 1Z0 2 4)
