摘要
目的用原子力显微镜(atom force microscope,AFM)观察汉坦病毒感染前后Vero-E6细胞的形貌变化。方法①用不同浓度的戊二醛固定细胞;②用不同稀释度的病毒感染细胞;③用同一稀释度的病毒分别感染细胞15、30、45、60、75、90分钟。结果①用0.5%和1.0%的戊二醛处理的细胞形态正常;用1.5%和2.0%的戊二醛固定的细胞表面凸凹不平孔洞较多,大部分细胞皱缩。②用不同稀释度的病毒感染过的Vero-E6细胞,与正常细胞相比,细胞表面粗糙,在细胞核周围有孔洞出现,但稀释度不同出现孔洞的数量也有差别,细胞表面光滑程度也有不同。③用同一稀释度的病毒感染细胞不同时间,发现细胞的形貌、表面粗糙度及细胞表面的孔洞数量都有了变化。结论用AFM技术可以观察到病毒感染前后细胞的超微结构,近似的反映出病毒感染细胞的动态过程。
Objective Apply AFM to observe the different cell surface between normal Veto-E6 cell and Vero-E6 cell contracted by Han taan virus. Methods ①Cells were treated with different concentration of glutaraldehyde;②Cells were treated with different dilution of Han taan virus ; ③Infect cells by the same dilution＇ s glutaraldehyde for 15,30,45,60,75, and 90 minutes respectively. Resuits ①Cells surface treated by 0.5% - 1.0% glutaraldehyde is normal, but the ones treated by 1.5% -2.0% glutaraldehyde have more protrusions and hollows, the majority of cells shrinkage; ②Veto-E6 cells surface infected by different dilution＇ s virus, compared with normal cells, is rougher and there are hollows arotmd nucleus, moreover, the hollow numbers and the smooth degree is also different as dilution concentration varying; ③The cell images, surface roughness, and hollow numbers also change when infected by the same dilution＇s virus for different time. Conclusion Applying AFM to observe cells can reveal the ultra micro-structure before and after cells are contracted by virus, reflect the dynamic process of virus infecting cells approximately.
出处
《中国实验诊断学》
2008年第5期598-601,共4页
Chinese Journal of Laboratory Diagnosis
