摘要
目的 评价中国人参对减少辐射诱导的人周围血 G0 期淋巴细胞 (PBL)微核量的作用。方法 采用细胞因子阻滞的微核法检测健康志愿者的血标本。体外 1 37Cs照射之前 ,每份血标本的单个核细胞培养以不同浓度 (0～ 2 0 0 0μg· m l- 1 )的人参根水提取物孵育 2 4 h。结果 (1)在 0 Gy时 ,每 10 0 0个双核 (BN )细胞的微核量是 11.3± 2 .7,人参水提取物≥ 5 0 0μg· ml- 1 时微核量下降 ,但微核量和人参水提取物的浓度之间的差异是显著的。 (2 )单独射线照射显著增加微核量 ,当暴露于 1Gy的照射时 ,随着培养介质中人参水提取物浓度的增加 ,微核量呈显著的线性降低 (R2 =0 .0 5 ,P =0 .0 0 2 ) ;对暴露于 1Gy辐射诱导的微核量 ,人参水提取物浓度在 15 0 0μg· m l- 1 时减少 4 6 .6 % ,浓度在 2 0 0 0μg·m l- 1时减少 5 7.6 % ;对暴露于 2 Gy辐射诱导的微核量的减少呈更强的线性相关 (R2 =0 .7,P=0 .0 0 0 1) ;(3)微核数据分析的结果呈现出与 Poisson模型相关的过度离散趋势。结果表明 :(1)人参水提取物浓度达 2 0 0 0 μg· ml- 1时 ,对 PBL 细胞毒作用无明显影响 ;(2 )人参水提取物浓度在 5 0 0 μg· ml- 1和 2 0 0 0 μg· ml- 1之间时 ,对抗 1 37Cs照射诱导的 MN发挥保护作用。
Objective To assess the effect of Chinese ginseng on modifying the radiation-induced micronuclei (MN) yield in human G 0 peripheral blood lymphocytes (PBL).Methods The cytokinesis-blocked MN assay was conducted in blood samples obtained from healthy volunteers (n = 4). Before 137 Cs ex vivo irradiation, mononuclear cell cultures from each sample were incubated 24 h with different concentrations (0 ~ 2000 μg·ml -1 ) of crude water extract of ginseng dry root. Results We found that (1) at 0 Gy, MN yield (mean ± SEM) was 11.3 ± 2.7 per 1000 binucleated (BN) cells. MN yield decreased with the presence of ginseng water extract concentrations ≥ 500 μg·ml -1, although the differences between MN yields and ginseng concentrations were insignificant; (2) radiation alone sharply increased the MN yields. At 1 Gy exposure, as ginseng water extract concentration increased in the culture, resulting in a strong linear decline of MN yields (R2 = 0.5, P = 0.002). The extent to which ginseng water extract reduced the MN yields induced by 1 Gy exposure, was 46.6% at 1500 μg·ml -1 and 57.6% at 2000μg·ml -1. At 2 Gy exposure, the trend of a linear decline in MN yield was even stronger(R2 = 0.7, P = 0.0001); and (3) analysis of the MN data suggested that there was a tendency for overdispersion relative to the Poisson model. Our results indicated that (1) ginseng water extract exerts no apparent cytotoxic effect on PBL at concentrations up to 2000μg·ml -1; (2) the protection of ginseng water extract against 137Cs-induced MN in human PBL is dose-dependent at concentration between 500 μg·ml -1 and 2000 μg· ml -1.Conclusion Ginseng may have therapeutic value as a possible radioprotector for normal tissue during radiotherapy of cancer patients.
出处
《新乡医学院学报》
CAS
2004年第3期153-159,共7页
Journal of Xinxiang Medical University
关键词
人参
辐射防护
微核
淋巴细胞
Ginseng
radioprotection
micronuclei
lymphocytes
