摘要
野败型细胞质雄性不育系统是选配杂交稻组合广泛应用的主要不育细胞质资源 ,野败型细胞质雄性不育的育性恢复能力由两个恢复基因控制。以前的研究表明 ,明恢 6 3具有 2个恢复基因Rf3和Rf (u) ,分别位于第 1和第 10染色体上。为了分别准确估计这两个恢复基因的遗传效应 ,根据分子标记基因型 ,从珍汕 97/明恢 6 3衍生的 2 4 1个F9重组自交系群体中选择两个自交系RI2 4和RI183,它们分别含有单个恢复基因Rf3和Rf (u) ,将RI2 4和RI183与珍汕 97A杂交 ,分别得到F1A和F1B,再自交得到F2A和F2B。在武汉和海南分别考察F1的育性 ,F1A的自然结实率海南和武汉分别为 5 3 4 %和 6 0 2 % ,F1B的自然结实率海南和武汉分别为 70 5 %和 75 7%。而珍汕 97A/明恢 6 3的杂种汕优 6 3结实率为 81 4 %。F2A和F2B群体育性分离均符合 1个主基因 1∶3的孟德尔期望分离比 ,表明 ,RI2 4和RI183分别只含有一个恢复基因Rf3和Rf (u) 。Rf(u) 的效应较大 ,恢复力强 ,它单独几乎可以使育性恢复正常。利用标记辅助选择方法 ,转移两个恢复基因可以快速选育优良恢复系。
Among various sources of cytoplasmic male sterility (CMS), wild abortive (WA) system is the predominantly used CMS source in hybrid rice breeding The fertility restoration ability of WA CMS system was reported to control by two restorer genes Our previous results showed there were two restorer genes Rf3 and Rf(u) in the restorer line located on chromosomes 1 and 10 respectively To accurately evaluate their restorability, two recombinant inbred lines (RI24 and RI183) separately contained a different restorer gene, respectively, were selected from a recombinant inbred line (RIL) population derived from an elite rice hybrid, Shanyou 63 in this study The two RIL lines were crossed with the sterile line of the cross, Zhenshan 97A, and respective hybrids F 1A and F 1B were obtained to test the fertility restorability of the individual restorer genes Comparison of fertility restorability were conducted between the restorer line Minghui 63 and the two lines The natural seed setting rates of F 1A and F 1B were 53 4% and 70 5% in Hainan, 60 2% and 75 7% in Wuhan, respectively While Shanyou 63 with the both restorer genes were 81 4% in Wuhan. Chi square test showed that the segregation of sterile to fertile in F 2A population and F 2B population were in agreement with the ratio of single Mendalian segregation, and indicated that RI24 and RI183 really possessed only one restore gene Rf3 and Rf(u) respectively. The restorability of Rf(u) was much stronger than that of Rf3 , and the Rf(u) alone could almost restore the fertility to a normal level as compared with the restorer line of Shanyou 63. The results implied that an elite restorer line could be rapidly developed through marker aided selection for the two restorer genes.
出处
《分子植物育种》
CAS
CSCD
2003年第4期497-501,共5页
Molecular Plant Breeding
基金
国家重点基础研究发展规划项目(G1998010200)的资助。
