摘要
选用矮败型细胞质(DA-CMS)的不育系协青早A(XqA)对8个携带供体恢复基因Rf3,14个携带供体恢复基因Rf4的水稻染色体单片段代换系(Chromosome single segment substitution lines,SSSLs)和华粳籼74(HJX74,Rf3Rf3/Rf4Rf4)的恢复力进行测交鉴定,并利用恢复力最强的SSSL S18与XqA组配,采用杂交回交和分子标记辅助选择的方法,构建BC3F2群体,筛选鉴定出携带基因型Rf3Rf3/Rf4Rf4,Rf3Rf3/rf4rf4,rf3rf3/Rf4Rf4和rf3rf3/rf4rf4的BC3F2单株,观察这些植株的花粉和小穗育性,并利用213个多态性SSR标记对这些单株进行遗传背景分析,研究恢复基因Rf3和Rf4对于DA-CMS的遗传效应。结果表明:在同一细胞核背景下,22个SSSLs和HJX74对于XqA的恢复力存在着显著的不同,携带恢复基因Rf3的SSSLs恢复力均低于携带恢复基因Rf4的SSSLs,并且低于对照品种HJX74;携带Rf3的SSSL S1和SSSL S2对于XqA的花粉(小穗)育性分别为14.1%(61.7%)和15.7%(62.5%),表现出较弱的恢复力;携带Rf4的SSSL S16-SSSL S22对于XqA的花粉和小穗育性均高于87%和95%,其中SSSL S18的表现出最强的恢复力(花粉育性89.2%,小穗育性96.4%)。在构建的BC3F2群体中,携带基因型Rf3Rf3/Rf4Rf4,Rf3Rf3/rf4rf4,rf3rf3/Rf4Rf4和rf3rf3/rf4rf4单株的遗传背景片段数平均为1.0,对应于恢复基因Rf3和Rf4座位的代换片段平均长度分别为18.3 cM和12.8 cM。这4种基因型的遗传效应大小为Rf3Rf3/Rf4Rf4>rf3rf3/Rf4Rf4>Rf3Rf3/rf4rf4>rf3rf3/rf4rf4,即恢复基因Rf4的恢复力大于Rf3的恢复力,而且这两个基因表现出累积效应。
For investigation of the restoring abilities of chromosome single segment substitution lines （SSSLs） in rice, 8 SSSLs carrying Rf3 gene from different donors, 14 SSSLs with Rf4 gene from different donors and Indica Huajingxian74 （ HJX74, Rf3Rf3/Rf4Rf4 ） were crossed to a DA-CMS line of XieqingzaoA （XqA）, and their phenotyping for pollen and spikelet fertility were evaluated. The SSSL S18 with the strongest restorering ability to XqA, was identified, and then the BC3 F2 population possessing the genetic background of the SSSL S18 was generated from the cross between the SSSL S18 and XqA by backcrossing and marker-assisted selection （MAS）. To analyze the genetic effects of the Rf genes for DA- CMS system, the BC3F2plants, carrying the genotypes Rf3Rf3/Rf4Rf4 ,Rf3Rf3/rf4rf4, rf3rf3/Rf4Rf4 and rf3rf3/rf4rf4, were selected by MAS and their genetic background were analyzed by using 213 SSR markers. The results were as follows. There were much differences in restoring abilities among the 22 SSSLs and HJX74. The restoration abilities of SSSLs carrying Rf3 gene were weaker than that of SSSLs with Rf4 gene and HJX74. SSSL S1 and SSSL $2 carrying the Rf3gene exhibited 14. 1% （61.7%） and 15.7% （62.5%） pollen （spikelet） fertility of F~ plants and possessed the weaker restoring ability to DA-CMS. Out of the fourteen SSSLs with Rf4 gene, high levels of pollen fertility （ 〉 87% ） and spikelet fertility （ 〉 95% ） were observed in the crosses of XqA/SSSL S16 - SSSL S22, which showed stronger restorer ability to DA-CMS. When 213 SSR markers were used to analyze the genetic background and average length substituted chromosome segments （SCS） of the BC3F2 individuals, the numbers of SCS carried by BC3F2 individuals, having the genotypes of Rf3Rf3/Rf4Rf4, Rf3Rf3/rf4rf4, rf3rf3/Rf4Rf4 and rf3rf3/rf4rf4, was l. 0, while the average lengths of the SCS, corresponding to Rf3 and Rf4 loci, were 18.3 cM and 12.8 eM, respectively. The genetic effects of the Rf genes on pollen and spikelet fertility would be Rf3Rf3/Rf4Rf4 〉 rf3rf3/Rf4Rf4 〉 Rf3Rf3/rf4rf4 〉 rf3rf3/rf4rf4, and the effect of the Rf4 appeared to be slightly larger than that of the Rf3, as well as their effects were additive for the CMS system.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第5期576-583,共8页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(30830074)
关键词
水稻
单片段代换系
质核互作雄性不育系
恢复力
Oryza sativa L.
Single segment substitution line （SSSL）
Cytoplasmic male sterility （CMS）
Restoringability
