摘要
背景:脑水肿使颅内压明显增高和神志障碍,严重可导致脑疝,这些病理变化是造成脑功能障碍的主要原因。应用三七皂甙治疗脑水肿是目前国内外研究热点,具有重大理论意义。目的:探讨三七皂甙对百日咳菌液致大鼠脑水肿的治疗作用及机制。设计:随机对照研究。地点和对象:实验地点:中南大学湘雅医学院机能实验室。健康SD大鼠40只,随机分为生理盐水组、百日咳菌液组、三七皂甙组、甘露醇组4组。干预:采用百日咳菌液注入左颈内动脉导致大鼠脑水肿。主要结局观察指标:各组大鼠左脑组织含水量、伊文思蓝、丙二醛水平、超氧化物歧化酶(superoxidedismutase,SOD)活性、肿瘤坏死因子α(tumornecrosisfactor-α,TNFα)水平。结果:左脑组织TNFα水平,三七皂甙组(457.9±92.6)pg/g明显低于百日咳菌液组(725.6±76.3)pg/g和甘露醇组(686.4±102.1)pg/g(q=3.52～4.18,P<0.05)。左脑组织的含水量:生理盐水组(78.8±0.3)%、三七皂甙组(79.8±0.4)%和甘露醇组(82.2±0.3)%均较百日咳菌液组(83.9±0.2)%为低(q=3.64～4.56,P<0.05),三七皂甙组较甘露醇组低(q=3.38,P<0.05)。左脑组织的伊文思蓝含量:生理盐水组(1.9±0.7)μg/g、三七皂甙组(5.2±0.9)μg/g和甘露醇组(8.4±0.6)μg/g均较百日咳菌液组(12.1±0.5)μg/g低(q=3.
BACKGROUNDS:The brain edema results in remarkable increasing of intracranial p ressure,mental disturbance and even cerebral hernia in the severe case.Those pat hological changes are known as the essential factors for the cerebral functional disturbance.Researches on the treatment of panax notoginseng saponin for brain edema is the hotspot at present at home and abroad with great significance theor etically. OBJECTIVE:To explore the treating action and mechanism of panax notoginseng sa ponin(PNGS) on rat brain edema induced by pertussis bacterial(PB) liquid. DESIGN:Randomized controlled study. SETTING and MATERIALS:The experiment was performed in the Function Laboratory in Xiangya Medical College of Central South University.40 normal SD rats were ra ndomized into 4 groups,namely normal saline(NS) group, PB group,PNGS group and m annitol(MN) group. INTERVENTION:PB was injected into the left internal carotid artery of the rat inorder to induce brain edema. MAIN OUTCOME MEASURES:The contents of water and Evans blue, the level of malon dialdehyde(MDA),superoxide dismutase(SOD) activity, and the level of tumor necro sis factor-α(TNFα) in the left brain tissue in each group. RESULTS:TNFαlevel in the left brain tissue in the PNGS group[(457.9±92.6)pg/ g]was remarkably lower than that in the PB group[(725.6±76.3) pg/g]and the MN g roup[(686.4±102.1) pg/g](q=3.52-4.18,P< 0.05)respectively.The water contents i n the NS group[(78.8±0.3)%,PNGS group[(79.8±0.4)%]and MN group[(82.2±0.3)% ] were lower than that in PB group[(83.9±0.2)%](q=3.64-4.56,P< 0.05) successi vely,and that in PNGS group was lower than that in MN group(q=3.38,P< 0.05).The contents of Evans blue in the NS group[(1.9±0.7) μg/g],PNGS group[(5.2±0.9) μg/g]and MN group[(8.4±0.6) μg/g]were lower than that in PB group[(12.1±0. 5) μg/g](q=3.82-4.45,P < 0.05) successively,which were lower in PNGS group as compared with MN group(q= 3.66,P< 0.05).The treatment reduced the level of MDA and increased SOD activity in each group(q=3.42-4.19,P< 0.05). CONCLUSION:PNGS plays a role in the treatment of rat brain edema induced by PB ,whose mechanism is related to lipid eroxydation inhibition and alleviating TNF αformation.
出处
《中国临床康复》
CSCD
2004年第13期2589-2591,共3页
Chinese Journal of Clinical Rehabilitation
