摘要
目的:探讨以具有增强免疫力、保护酒精性肝损伤、提高缺氧耐受力、抗疲劳等功效的三七为主要成分,以茶作为载体的三七袋泡茶对正常小鼠和2型糖尿病模型小鼠血糖及体质量的影响。方法:实验于2005-01/02在广西区疾病预防控制中心保健食品功能学检验鉴定国家认可实验室完成。选用SPF级昆明种雄性小白鼠150只。①随机选取124只小鼠禁食24h后给予四氧嘧啶(65mg/kg)一次腹腔注射造模,6d后100只小鼠造模成功(空腹血糖10～25mmol/L)。②降低空腹血糖试验:取造模成功52只鼠做降低空腹血糖试验,随机分为4个组:三七袋泡茶低、中、高剂量组和模型组,每组13只。三七袋泡茶低、中、高剂量组:分别灌胃375,750,1500mg/kg剂量(分别相当于最小人体推荐用量的5,10,20倍)的三七袋泡茶[由云南某(集团)有限公司提供,有效成分为三七皂甙,含量为10%;批号:20040801,规格为1.5g/袋;人体推荐用量为75mg/kg]。实验前每次称取100g样品加入1L(85℃)蒸馏水浸泡,过滤后取滤液后再用1L(85℃)蒸馏水浸泡,然后将2次浸泡液合并,于沸水浴中低温(60℃)减压浓缩至100mL,用蒸馏水将其分别配成供试液],灌胃量0.002mL/kg。模型组:灌胃等量蒸馏水。1次/d,连续30d后检测禁食4h后的空腹血糖。比较各组动物空腹血糖及血糖下降百分率。血糖下降百分率=(实验前血糖值-实验后血糖值)/实验前血糖值×100%。③葡萄糖耐量试验:将其余48只造模成功小鼠随机分为4组:分组情况同“降低空腹血糖试验”,每组12只。小鼠分组后禁食4h,各组干预措施同“降低空腹血糖试验”,20min后各组灌胃2.0g/kg葡萄糖,测定给葡萄糖后0,0.5,2h血糖值,观察各组灌胃葡萄糖后各时间点血糖曲线下面积的变化,血糖曲线下面积=0.25×(0h血糖值+4×0.5h血糖值+3×2h血糖值)。④正常小鼠空腹血糖测定实验:将未造模的正常小鼠26只随机分为2组:三七袋泡茶高剂量组(灌胃三七袋泡茶1500mg/kg,灌胃量0.002mL/kg)和对照组(灌胃等量蒸馏水),每组13只。灌胃1次/d,连续30d后测定其禁食4h后的血糖值。⑤各实验均于实验开始及结束时称体质量。⑥计量资料差异比较采用单因素方差分析。结果:小鼠126只均进入结果分析。①各组小鼠实验结束时体质量均有所增加,但与实验前比较,差异不明显(P>0.05)。②实验开始与实验30d后,三七袋泡茶高剂量组与对照组的正常小鼠的空腹血糖比较,差异不明显(P>0.05)。③给药30d后各剂量给药组小鼠空腹血糖明显低于模型组(P<0.05),且三七袋泡茶高剂量组的血糖下降百分率明显高于模型组(P<0.05)。④三七袋泡茶高、中剂量组小鼠血糖曲线下面积明显低于模型组(P<0.01,0.05)。结论:①三七皂甙袋泡茶对2型糖尿病模型和正常小鼠体质量无明显影响。②三七皂甙袋泡茶可降低2型糖尿病模型小鼠血糖,但对正常小鼠空腹血糖无明显影响。
AIM： To explore the effect of sanchi tea bag with tea as carrier characterized by improving immunity, protecting alcoholic hepatic injury, increasing anoxic endurance and relieving fatigue on blood glucose and body mass of normal mice and model mice with type 2 diabetes.
METHODS： The experiment was carried in Health Food Functional Assessment Laboratory of Center for Disease Prevention and Control in Guangxi from January to March 2005. Totally 150 Kunming male mice of SPF grade were selected in this study. ① Totally 124 mice were injected with 65 mg/kg alloxan in abdominal cavity after 24-hour fasting. Totally 100 mice were successful model in result （10-25 mmol/L fasting blood glucose）.② Test of fasting blood glucose： 52 mice were treated with test of fasting blood glucose and randomly divided into 4 groups： low, middle and high dosage of sanchi tea bag groups and model group with 13 in each group. Low, middle and high dosage of sanchi tea bag groups： Mice were perfused with 375, 750 and 1 500 mg/kg sanchi tea bag respectively （equal to 5, 10 and 20 times of recommending dose of minimal human body; provided by a .limited company in Yunnan; sanchi saponin as the effective component; volume of 10%; batch number： 20040801; 1.5 g/bag; 75 mg/kg as recommending dose of human body）. Before experiment, 100 g sample was dipped in 1 L 85 ℃ distilled water, after filtrated, it was dipped in another 1 L 85 ℃ distilled water, and then was filtrated. All filtrate was concentrated to 100 mL in boiling water with reduction pressure and low temperature （60 ℃ ）, then it was confected into 3 concentration solutions with distilled water. The volume was 0.2 mL/10 g. Model group： Mice were perfused with the same volume of distilled water once a day. After 30 days, the fasting blood glucose was assayed after 4 hours. The fasting blood glucose and decreasing ratio were compared between groups. Decreasing ratio of blood glucose = （blood glucose before experiment-bloed glucose after experiment）/bloed glucose before experiment x 100%. ③ Test of glucose endurance： The other 48 mice were divided into 4 groups with 12 in each group according to decreasing test of fasting blood glucose. After fasting 4 hours, mice were treated as the same as decreasing test of fasting blood glucose. Twenty minutes later, mice in each group were perfused with 2.0 g/kg glucose to assay value of blood glucose within 0, 0.5 and 2 hours respectively. The areas under blood glucose curve were compared. The area under blood sugar curve =0.25 ×（value of blood glucose in zero hour ＋ 4 × value of blood glucose in half hour ＋ 3 ×value of blood glucose in two hours）. ④Test of fasting blood glucose of normal mice： 26 normal mice were divided into 2 groups： high dose of sanchi tea bag group （perfused with 1 500 mg/kg sanchi tea bag, volume of 0,2 mL/10 g） and control group （perfused with the same volume of distilled water） with 13 in each group. Mice were perfused once a day for 30 days, then value of blood glucose 4 hours after fasting was assayed, ⑤ All mice were weighed at the beginning and the end of test.⑥Measurement data were compared with single factor analysis of variance,
RESULTS： Totally 126 mice entered result statistics, ① The body weights of mice were increased at the end of test, but there were not significantly difference as compared with those before experiment （P 〉 0.05）.② There was no difference in blood glucose between high dose of sanchi tea bag group and control group at the beginning and 30 days after experiment （P 〉 0.05）. ③ After 30-day medication, the value of fasting blood glucose in each dose group were significantly lower than that in model group （P 〈 0.05）, and the decreasing ratio in high dose group was significantly higher than that in model group （P 〈 0.05）. ④ The areas under blood glucose curve of high dose group and middle dose group were significantly lower than that in model group （P 〈 0.01, 0.05）. CONCLUSION： ① Sanchi tea bag dose not have effect on body mass of model mice with type 2 diabetes and normal mice. ② Sanchi tea bag can decrease blood glucose of model mice with type 2 diabetes, but dose not have effect on fasting blood glucose of normal mice.
出处
《中国临床康复》
CSCD
北大核心
2006年第11期84-86,共3页
Chinese Journal of Clinical Rehabilitation
