摘要
对长寿花茎段培养 7～ 8d ,部分茎段腋芽萌动。 30d培养结果表明 ,芽诱导的最佳培养基是 :MS +6 -BA1 0mg/L +NAA0 5mg/L ;芽伸长的最佳培养基是 :1/ 4MS ;形成有效节的最佳培养基是 :MS。继代培养中各培养基上无叶茎段均较带叶茎段萌芽率高。在 5、 6、 7号培养基上 ,叶片培养 9d时 ,从叶柄基部开始形成不定芽 ,在其它培养基上 ,14d才开始有不定芽形成。 30d培养结果还表明 ,诱导叶片再生不定芽的最佳培养基是 :1/ 2MS +6 -BA1 0mg/L。诱导根生长的最佳培养基是 :1/
Having cultured the stem sections of Kalanchoe blossfeldiana in vitro for 7～8days, the axillary buds of explants can germinate. The result of culturing for 30 days showed that the most suitable medium to induce buds was MS+6-BA1.0+NAA0.5 mg/l, the most suitable one to make the bud stretch was 1/4 MS, and toform available knots was MS. During the continuing culture, the germinating rate of stem sections with no leaves was higher than that of the sections with leaves on all the above media. Adventitious buds began to form at the base of petiole after having been cultured for 9 days on the media No.5,6,7. On the other media, however, they only began to form after being cultured for 14 days. Simultaneously, the most suitable medium to induce the leaves to regenerate adventitious buds was 1/2 MS+6-BA1.0 mg/l, and to induce the roots to grow was 1/4 MS.
出处
《中国农学通报》
CSCD
2004年第2期12-14,共3页
Chinese Agricultural Science Bulletin
关键词
长寿花
离体培养
植株再生
外植体
培养基
肉质植物
Kalanchoe blossfeldiana
Vitro culture and plantlet regeneration
Influence factors
